| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | Recombinant protein with human Ki67 (Position: K2860-I3256) was used as the immunogen for the Ki-67 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
Ki-67 Antibody / MKI67 is a anti-KI-67 Rabbit antibody Recombinant Rabbit Monoclonal clone 31K02 supplied in Liquid format. Recommended for workflows such as Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC) with listed reactivity in Human, Mouse, Rat. Reported localization: Nuclear.
Key elements and design rationale
- Target: KI-67
- Antibody details: Rabbit, Recombinant Rabbit Monoclonal, clone 31K02, isotype Rabbit IgG
- Format: Liquid
- Applications (as listed): IHC, IF, ICC/IF
Biological background
Ki-67 is involved in ribosomal RNA transcription and organization of the nucleolus during interphase. During mitosis, it localizes to the perichromosomal layer, forming a structural scaffold that helps protect chromosomes and maintain proper segregation. Studies using Ki-67 antibody have revealed that depletion of this protein disrupts chromosomal condensation and segregation, underscoring its functional roles beyond serving as a proliferation marker. These insights have expanded its importance from being a passive marker of division to an active participant in cell cycle regulation.
In cancer biology, Ki-67 is one of the most important clinical biomarkers. Tumor samples are routinely stained with Ki-67 antibody to determine the proliferation index, which correlates with tumor grade, aggressiveness, and prognosis. High Ki-67 labeling indices are observed in fast-growing tumors such as breast, prostate, and brain cancers, and are associated with poor outcomes. Conversely, lower Ki-67 indices are linked to slower-growing neoplasms and better survival rates. Pathologists use Ki-67 antibody immunohistochemistry to quantify nuclear staining percentages in tumor biopsies, providing valuable prognostic information.
Beyond oncology, Ki-67 antibody is applied in regenerative biology and developmental studies. Since this protein marks actively dividing cells, it is used to track stem cell proliferation, tissue regeneration, and embryonic development. Immunofluorescence assays with Ki-67 antibody help identify proliferative niches in tissues such as bone marrow, gut, and brain. Flow cytometry can also measure Ki-67 expression at the single-cell level, making it useful in studies of immune activation, hematopoietic stem cell dynamics, and vaccine responses.
By supplying validated Ki-67 antibody reagents,
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Monoclonal antibodies provide a defined epitope recognition profile that can support consistent comparisons across experiments.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
