| Field | Specification |
|---|---|
| Alternative Names | Kinesin-like protein KIF15|Kinesin-like protein 2 (hKLP2)|Kinesin-like protein 7|Serologically defined breast cancer antigen NY-BR-62|KIF15|KLP2|KNSL7 |
| Clonality | |
| Form | Liquid |
| Gene ID | |
| Host | |
| Immunogen | kinesin family member 15 |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Product Overview
KIF15, also named as KLP2 and KNSL7, belongs to the kinesin-like protein family and KLP2 subfamily. It is a plus-end directed kinesin-like motor enzyme which involved in mitotic spindle assembly. Several isoforms of KIF15 exist due to the alternative splicing, with molecular weight between 130-160 kDa. Sometimes higher molecular weight around 200 kDa can also be observed, which may be a modified variant of KIF15.
Polyclonal Rabbit AntibodyValidated Applications
| Application | Recommended Dilution |
|---|---|
| ELISA | Refer to datasheet |
| Western Blot | Refer to datasheet |
| Immunohistochemistry | Refer to datasheet |
| Immunofluorescence | Refer to datasheet |
| Immunoprecipitation | Refer to datasheet |
Immunogen & Specificity
Immunogen: kinesin family member 15
UniProt/Swiss-Prot: Q9NS87
Product Specifications
| Host Species | Rabbit |
|---|---|
| Clonality | Polyclonal |
| Isotype | IgG |
| Species Reactivity | Human, Mouse, Rat |
| Purification Method | Immunogen Affinity Purified |
| Purity | ≥95% as determined by SDS-PAGE |
| Molecular Weight | 160 kDa |
| Storage | PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.) |
Reactivity Notes
Confirmed reactivity in Human, Mouse, Rat samples; validated for ELISA, Western Blot, Immunohistochemistry, Immunofluorescence, Immunoprecipitation.
Safety & Handling
- For Research Use Only (RUO). Not intended for diagnostic procedures.
- Shipping: cold-chain shipment (typically with ice packs).
- Upon receipt: store at recommended temperature as soon as possible.
- Sales terms and conditions: Please review prior to ordering.
Related Products
Explore additional antibodies and related detection reagents for KIF15 in the BioHippo catalog.
KIF15 antibody
First, usually it's not suggested to recycle antibodies. After use, buffer system of antibodies has changed. The storage condition of recycled antibodies for different customers also varies. Thus, the performance efficiency of recycled antibodies can’t be guaranteed. Besides, FineTest ever conducted the antibody recycling assay. Assay results show recycling times of different antibodies also varies. Usually, higher antibody titer allows more repeated use. Customers can determine based on experimental requirements.
Notes: After incubation, we recycle rest antibodies to centrifuge tube and store at 4℃. High titer antibodies can be stored for a minimum of one week. Reuse about three times.
Components of FineTest antibody buffer are usually PBS with proclin300 or sodium azide, BSA, 50% glycerol. Common preservative is proclin300 or sodium azide, which is widely applied in the lab and industry.
Most antibodies are stored at -20℃. Directly-labeled flow cytometry antibodies should be stored at 2 - 8℃. The shelf life is one year. If after sales issues for purchased antibodies appear, return or replacement is available. Usually, antibodies can be still used after the one-year warranty. We can offer technical support services.
Directly-labeled flow cytometry antibodies are ready-to-use without dilution. Other antibodies are usually concentrated. Follow the dilution ratio suggested in the manual. Dilute solution for different experiments also varies. Common antibody dilution buffers are acceptable(e.g. PBST, TBST, antibody blocking buffer).
Common retrieval buffers: Tris-EDTA Buffer(pH 9.0); Citrate Buffer(pH 6.0)
Heat induced antibody retrieval:
Method 1: Water-bath heating: Put the beaker with retrieval buffer and slide in the boiling water bath. Keep the boiling state for 15min. Naturally cool to room temperature;
Method 2: Microwave retrieval: Put the beaker with retrieval buffer and slide in the microwave oven. Heat at high power for 5min, Switch OFF for 3min, Heat at medium power for 5min. Naturally cool to room temperature.
BioHippo can support custom antibody formatting and service options for selected products. Contact us to discuss your project requirements.
Potential customization options include:
- Custom conjugation: fluorophore, biotin, enzyme, or nanoparticle labeling available on request for qualified antibodies.
- Bulk or scale-up quantities: volume pricing and custom lead time estimates available for larger orders.
- Alternative formulations: azide-free, carrier protein-free, or lyophilized formats available upon request for selected products.
- OEM and private-label supply: custom packaging and branding available for commercial applications.
Submit your project specifications via the product contact form or reach us at support@ebiohippo.com for a tailored quotation.
Knockdown of KIF15 suppresses proliferation of prostate cancer cells and induces apoptosis through PI3K/Akt signaling pathway
IF: 7 Journal: Cell Death Discovery Author: Department of Urology, Shanghai General Hospital, Shanghai Jiaotong University School of Medicine, 200080, Shanghai, China Cited Date: 2023-09-08
KIF15 knockdown inhibits colorectal cancer proliferation and migration through affecting the ubiquitination modification of NRAS
IF: 6.16 Journal: American Journal of Cancer Research Author: Department of Colorectal Surgery, Fudan University Shanghai Cancer Center, 270 Dong’an Road, Shanghai 200032, China Cited Date: 2023-11-24
GSG2 (Haspin) promotes development and progression of bladder cancer through targeting KIF15 (Kinase-12)
IF: 4.831 Journal: AGING Cited Date: 2020-10-15
KIF15 knockdown suppresses gallbladder cancer development
IF: 4.492 Journal: European Journal of Cell Biology Cited Date: 2021-11-26
KIF15 promotes the development and progression of chordoma via activating PI3K-AKT signalling pathway
IF: 4 Journal: Heliyon Author: First Affiliated Hospital of Shenzhen University/Shenzhen Second People's Hospital, Shenzhen, Guangdong, 518000, China Cited Date: 2024-04-19
KIF15 facilitates gastric cancer via enhancing proliferation, inhibiting apoptosis, and predict poor prognosis
IF: 3.439 Journal: Cancer Cell International Cited Date: 2020-04-15
Identification of KIF15 as a potential therapeutic target and prognostic factor for glioma
IF: 2.301 Journal: Oncology Reports Cited Date: 2020-02-21
Knockdown of Kinase Family 15 Inhibits Cancer Cell Proliferation In vitro and its Clinical Relevance in Triple-Negative Breast Cancer
IF: 2.254 Journal: Current Molecular Medicine Cited Date: 2019-05-20
KIF15, a key regulator of nasopharyngeal carcinoma development mediated by the P53 pathway.
IF: 1.11 Journal: BIOCELL Author: Department of Otolaryngology & Head and Neck, The First Affiliated Hospital of Guangxi Medical University, Nanning, 530021, China Cited Date: 2023-06-09
KIF15 Accelerated The Progression of Nasopharyngeal Carcinoma and Predict Poor Prognosis
IF: Journal: Research Square Cited Date: 2021-04-23
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