| Field | Specification |
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| Immunogen | A synthetic peptide corresponding to a sequence in the middle region of human KIF7 was used as the immunogen for the KIF7 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
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| Target | |
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Overview
KIF7 Antibody / Kinesin-like protein KIF7 is a anti-KIF7 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB) with listed reactivity in Human.
Key elements and design rationale
- Target: KIF7
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB
Biological background
KIF7 is encoded by the KIF7 gene located on human chromosome 15q26.1. The protein is approximately 151 kilodaltons and contains an N-terminal motor domain, a coiled-coil stalk, and a C-terminal tail region required for signaling complex assembly. KIF7 localizes to the primary cilium and functions as a scaffold controlling the balance between active and repressive forms of the transcription factor GLI in response to Hedgehog pathway activation.
The KIF7 antibody detects a 134 kilodalton protein by western blot and reveals distinct ciliary tip staining by immunofluorescence. KIF7 regulates microtubule stability and controls ciliary length, essential for proper Hedgehog signal transduction. It restrains GLI2 and GLI3 activity by preventing excessive microtubule polymerization at the ciliary tip and recruiting pathway regulators such as SUFU.
Loss-of-function mutations in KIF7 cause ciliopathies, including Joubert syndrome and hydrolethalus syndrome, characterized by brain malformations and defective patterning during embryogenesis. Aberrant KIF7 signaling disrupts tissue polarity and organ development, while partial reduction contributes to tumorigenesis by allowing uncontrolled Hedgehog pathway activation.
KIF7 is an essential modulator of ciliary signaling and structural organization, integrating microtubule mechanics with developmental cues.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
