| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | CD158 antigen like family member B2; CD158 antigen-like family member B2; CD158b; CD158b2; GL183; KI2L3_HUMAN; Killer cell immunoglobulin like receptor two domains long cytoplasmic tail 3; Killer cell immunoglobulin-like receptor 2DL3; Killer inhibitory receptor cl 2-3; Killer inhibitory receptor CL2 3; KIR 023GB; KIR-023GB; KIR-K7b; KIR-K7c; KIR023GB; KIR2DL3; KIR2DS5; KIRCL23; MHC class I NK cell receptor; Natural killer associated transcript 2; Natural killer cell inhibitory receptor KIR2DL3; Natural killer-associated transcript 2; NKAT; NKAT-2; NKAT2; NKAT2a; NKAT2b; p58; p58 natural killer cell receptor clone CL 6; p58 natural killer cell receptor clone CL-6; p58 NK Receptor; p58 NK receptor CL 6; p58 NK receptor CL-6; p58.2 MHC class I specific NK receptor; p58.2 MHC class-I-specific NK receptor |
| Clonality | |
| Conjugate | |
| Form | Liquid |
| Host | |
| Immunogen | Fusion protein corresponding to a region derived from internal residues of Human killer cell immunoglobulin-like receptor, two domains, long cytoplasmic tail, 3/1/4/ short cytoplasmic tail, 4 |
| Isotype | |
| Product Type | |
| Reactivity | |
| Source | This product is a polyclonal antibody purified from rabbit antiserum. |
| Storage | |
| Target | |
| UniProt # |
Overview
This is a polyclonal anti-KIR2DL3 antibody raised in Rabbit, with confirmed utility in ELISA, IHC. It is designed to detect KIR2DL3 protein in Human, Mouse, Rat and supports researchers working in immunology contexts.
Key elements and design rationale
- Immunogen: Fusion protein corresponding to a region derived from internal residues of Human killer cell immunoglobulin-like receptor, two domains, long cytoplasmic tail, 3/1/4/ short cytoplasmic tail, 4 — determines the epitope region; confirm compatibility with sample preparation and expected post-translational modifications.
- Host species (Rabbit): Requires anti-rabbit-IgG secondary reagents for indirect detection.
- Polyclonal format: Recognizes multiple epitopes for robust signal; inherent lot-to-lot variability requires appropriate controls.
- Isotype (IgG): Matched secondary antibodies and isotype controls required. Compatible with standard Protein A/G purification workflows.
- Purification (Antigen affinity purification): Enriches for specific immunoglobulin classes; reduces non-specific background vs. crude antisera.
Biological background
KIR2DL3 (also referred to as CD158 antigen like family member B2, CD158 antigen-like family member B2, CD158b, CD158b2, GL183, KI2L3_HUMAN, Killer cell immunoglobulin like receptor two domains long cytoplasmic tail 3, Killer cell immunoglobulin-like receptor 2DL3, Killer inhibitory receptor cl 2-3, Killer inhibitory receptor CL2 3, KIR 023GB, KIR-023GB, KIR-K7b, KIR-K7c, KIR023GB, KIR2DL3, KIR2DS5, KIRCL23, MHC class I NK cell receptor, Natural killer associated transcript 2, Natural killer cell inhibitory receptor KIR2DL3, Natural killer-associated transcript 2, NKAT, NKAT-2, NKAT2, NKAT2a, NKAT2b, p58, p58 natural killer cell receptor clone CL 6, p58 natural killer cell receptor clone CL-6, p58 NK Receptor, p58 NK receptor CL 6, p58 NK receptor CL-6, p58.2 MHC class I specific NK receptor, p58.2 MHC class-I-specific NK receptor) is a protein target studied in Human systems. Expression, subcellular localization, and post-translational modifications vary across cell types and disease states — factors critical to antibody-based detection design. Consult UniProt, NCBI Gene, and primary literature for current annotation of KIR2DL3 biology in immunology.
Common research applications
- ELISA: Quantification of soluble target in biological fluids or culture supernatants. Ensure samples are within the linear detection range.
- IHC: Visualization of target in FFPE or frozen tissue sections. Optimize antigen retrieval and secondary detection for each tissue type.
Notes for experimental interpretation
- Isotype controls: Use an isotype-matched (IgG from Rabbit) control at equivalent concentration to assess non-specific background.
- Cross-reactivity: Polyclonal preparations may cross-react with related proteins. Orthogonal validation (siRNA, KO lysate) is recommended.
- Matrix effects: Sample matrix can affect performance; pilot dilution linearity and spike-recovery experiments are advised for quantitative studies.
- Species reactivity: Confirmed for Human, Mouse, Rat. Extrapolation to untested species requires empirical validation.
This product is a polyclonal antibody purified from rabbit antiserum.
KIR2DL3 is a protein target in Human, Mouse, Rat biology. This polyclonal antibody raised in Rabbit is designed to detect KIR2DL3 in ELISA, IHC applications, with IgG isotype.
Reported reactive against Human, Mouse, Rat. Immunogen derived from Human. Cross-reactivity with other species should not be assumed without documented data or empirical testing.
Reported for ELISA, IHC. Each format requires independent dilution optimization and appropriate controls. Unlisted applications require empirical testing.
This non-conjugated IgG antibody requires a compatible secondary. Select anti-rabbit IgG secondary conjugated to your preferred reporter (HRP, AP, fluorophore, or biotin), matched to IgG.
(1) Isotype control — IgG from Rabbit at matching concentration; (2) Positive control — known KIR2DL3-expressing cell/tissue; (3) Negative control — knockdown/knockout sample for specificity; (4) Dilution linearity — verify proportional signal decrease in your matrix.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
