KLK2 Antibody / Kallikrein 2

Overview

KLK2 Antibody / Kallikrein 2 is a research-use antibody directed against KLK2. It is supplied for use in common immunoassay contexts such as WB, IHC-P, ELISA (RUO).

Key elements and design rationale

• Target: KLK2.
• Description (provided): KLK2 (KALLIKREIN 2), also called GLANDULAR or PROSTATIC, is a protein that in humans is encoded by the KLK2 gene, and is particularly associated with prostatic tissue.
• Antibody type: Rabbit, Polyclonal (rabbit origin), Rabbit IgG.
• Format: Antigen affinity purified; Antigen affinity purified.
• Reported/predicted localization: Cytoplasmic.
• Species reactivity: tested: Human.
• Immunogen (if provided): A recombinant human partial protein corresponding to amino acids I25-P261 was used as the immunogen for the KLK2 antibody..

The information above helps you match the antibody format to your assay context, interpret species-dependent differences, and anticipate how epitope context (isoforms, PTMs, or conformational state) may influence signal.

Biological background

KLK2 (KALLIKREIN 2), also called GLANDULAR or PROSTATIC, is a protein that in humans is encoded by the KLK2 gene, and is particularly associated with prostatic tissue. The KLK2 is a member of glandular kallikrein gene family that comprises 25 to 30 highly homologous genes that encode specific proteases involved in the processing of biologically active peptides. The KLK2 gene is mapped to 19q13.33.And the KLK2 gene contains 5 exons. An alternative KLK2 transcript, which they call KLK2-linked molecule (KLM), that arises from the use of an alternate donor site within intron 1.KLM shares only the N-terminal 15-amino acid signal peptide with the original KLK2 protein; the mature proteins display no similarity.

For curated annotations (gene/protein naming, domains, isoforms, and pathway links) for KLK2, consult primary databases such as UniProt, NCBI Gene, and Ensembl.

Research relevance and current trends

• Context-dependent expression studies: researchers often examine KLK2 abundance and localization across perturbations (genetic, pharmacologic, or environmental) to connect phenotype to molecular changes.
• Reagent reproducibility: there is growing emphasis on antibody specificity checks using orthogonal approaches (e.g., genetic perturbation or independent antibodies) and transparent reporting of clone/lot information.
• Multi-modal datasets: antibody-based readouts are increasingly combined with transcriptomics and imaging to relate protein-level measurements to cell-state transitions.

Common research applications

• Western blotting (immunoblot) for relative detection of target protein abundance and apparent molecular weight.
• Immunohistochemistry for spatial mapping of target expression across tissues and cell types.
• ELISA-based detection or quantification in research assays (format- and epitope-dependent).

When comparing conditions, interpret changes in signal in the context of sample composition, expected localization, and any known isoform complexity for the target.

Notes for experimental interpretation

• Isoforms and PTMs: alternative splicing or post-translational modifications can change epitope accessibility and apparent molecular weight; interpret bands/signals accordingly.
• Cross-reactivity and matrix effects: background binding can vary by sample type, species, and blocking/detection chemistries; include appropriate negative controls.
• Control concepts: where feasible, use genetic perturbation (KO/KD/overexpression), orthogonal assays, or independent antibodies to support specificity claims.

Antibody considerations: Polyclonal reagents may recognize multiple epitopes and can increase sensitivity but may show broader binding profiles, while monoclonal clones provide a single-epitope readout that can improve consistency across experiments. If a conjugate is listed, the antibody supports more direct detection workflows; otherwise, it is typically used with a compatible secondary antibody.

Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.