| Field | Specification |
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| Mfr No | |
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| Host | |
| Immunogen | A synthesized peptide derived from human Cytokeratin 20 was used as the immunogen for the KRT20 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
KRT20 Antibody / Cytokeratin 20 / CK20 is a anti-KRT20 Rabbit antibody Recombinant Rabbit Monoclonal clone CDI-11 supplied in Liquid format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), Flow cytometry (FACS) with listed reactivity in Human, Mouse, Rat. Reported localization: Cytoplasm.
Key elements and design rationale
- Target: KRT20
- Antibody details: Rabbit, Recombinant Rabbit Monoclonal, clone CDI-11, isotype Rabbit IgG
- Format: Liquid
- Applications (as listed): WB, IHC, ICC, IF, IP, FACS
Biological background
Structurally, CK20 is a 46 kDa cytoskeletal protein composed of a central alpha-helical rod domain flanked by non-helical head and tail domains, allowing filament assembly with type II keratins such as KRT8. CK20 belongs to the cytokeratin family of intermediate filaments that provide mechanical resilience and maintain epithelial integrity. Co-localization studies show CK20 distributed in the cytoplasm and perinuclear region, forming filamentous networks characteristic of simple epithelia.
Functionally, CK20 supports epithelial cell shape, polarity, and resistance to mechanical stress. It interacts with desmosomal and hemidesmosomal components to anchor the cytoskeleton to cell junctions, maintaining tissue architecture. CK20 also participates in cellular differentiation programs, serving as a marker of terminal differentiation in gastrointestinal epithelia and Merkel cells. In the urothelium, CK20 expression increases during tissue repair and regeneration.
Clinically, CK20 serves as an important immunohistochemical marker in cancer diagnostics. It is strongly expressed in colorectal adenocarcinomas, transitional cell carcinomas of the bladder, and some gastric cancers, while absent in most lung, breast, and prostate carcinomas. Combined with CK7 expression patterns, CK20 staining aids in identifying metastatic tumor origin. Dysregulation of KRT20 gene expression has been linked to epithelial barrier dysfunction and tumor progression. Pathway involvement includes cytoskeletal organization, epithelial differentiation, and stress response signaling.
Immunohistochemical staining using KRT20 antibody reveals strong cytoplasmic filamentous localization in gastrointestinal and urothelial epithelia. The KRT20 antibody from
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Monoclonal antibodies provide a defined epitope recognition profile that can support consistent comparisons across experiments.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
