| Field | Specification |
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| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | A synthesized peptide derived from human CD223 was used as the immunogen for the LAG-3 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
LAG-3 Antibody / Lymphocyte activation gene 3 protein / CD223 is a anti-LAG-3 Rabbit antibody Recombinant Rabbit Monoclonal clone 31L58 supplied in Liquid format. Recommended for workflows such as Immunofluorescence (IF), Immunohistochemistry (IHC), Immunocytochemistry (ICC) with listed reactivity in Human.
Key elements and design rationale
- Target: LAG-3
- Antibody details: Rabbit, Recombinant Rabbit Monoclonal, clone 31L58, isotype Rabbit IgG
- Format: Liquid
- Applications (as listed): IF, IHC, ICC
Biological background
LAG-3 antibody is widely applied in immunology, oncology, and translational research. In cancer, LAG-3 expression marks exhausted T cells within the tumor microenvironment. In chronic infection, sustained LAG-3 expression dampens antiviral responses. By detecting LAG-3, researchers can evaluate immune exhaustion, tolerance mechanisms, and therapeutic checkpoint blockade strategies.
Flow cytometry with LAG-3 antibody identifies exhausted or regulatory T cell subsets. Immunohistochemistry maps LAG-3 expression in tumors and inflamed tissues, while immunofluorescence highlights surface distribution on lymphocytes. Western blotting confirms expression in activated immune cell lysates. These methods enable detailed characterization of immune checkpoint biology.
LAG-3 has therapeutic relevance as a target for immune checkpoint inhibitors. Antibodies blocking LAG-3 restore T cell function and enhance antitumor immunity. Clinical trials are evaluating LAG-3 blockade alone and in combination with PD-1 inhibitors. By applying LAG-3 antibody, scientists can monitor expression as a biomarker for patient selection and therapeutic response.
LAG-3 is also involved in autoimmune disease and transplantation. It modulates T regulatory cell function and dendritic cell activation, influencing tolerance and graft survival. Studying LAG-3 with antibody based methods provides insights into balancing immunity and tolerance in clinical settings.
LAG-3 antibody from
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Monoclonal antibodies provide a defined epitope recognition profile that can support consistent comparisons across experiments.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
