| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human Lamin B2/LMNB2 recombinant protein (Position: L128-E585) was used as the immunogen for the Lamin B2 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
Lamin B2 Antibody / LMNB2 is a anti-B2 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), ELISA with listed reactivity in Human, Rat. Reported localization: Nucleus lamina.
Key elements and design rationale
- Target: B2
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, IHC, ELISA
Biological background
Lamin B2 is expressed in most somatic tissues and plays particularly important roles in proliferating cells. Mutations in LMNB2 have been associated with neurological disorders such as progressive myoclonus epilepsy, underscoring the importance of Lamin B2 for brain development and function. Studies have also implicated changes in lamin expression in processes such as aging, cancer, and viral infection, making antibodies against these proteins highly valuable for mechanistic investigations. The Lamin B2 antibody provides a reliable means of probing nuclear envelope biology and identifying changes that accompany disease progression.
In experimental applications, the Lamin B2 antibody has broad utility. Western blotting can reveal protein levels and detect shifts in size due to post-translational modifications. Immunofluorescence microscopy allows visualization of nuclear lamina organization and potential defects in nuclear envelope integrity. Immunohistochemistry enables tissue-level assessment of lamin expression, which may vary between normal and diseased tissues. Given lamin involvement in DNA replication and repair, the Lamin B2 antibody is also relevant in studies of genomic stability and cellular responses to stress. These uses highlight the versatility of the reagent for both basic and translational research.
The structural role of lamin proteins makes them targets of interest in cancer research. Altered lamin expression has been linked to changes in nuclear morphology, a hallmark of many tumor cells. Lamin B2 dysregulation could influence tumor progression by affecting nuclear stiffness, gene expression, and mechanotransduction.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
