| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human Galectin-3/LGALS3 recombinant protein (Position: R129-I250) was used as the immunogen for the LGALS3 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
LGALS3 Antibody / Galectin 3 / GAL3 is a anti-LGALS3 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), ELISA with listed reactivity in Human, Mouse, Rat.
Key elements and design rationale
- Target: LGALS3
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, ICC, IF, ELISA
Biological background
Galectin-3 is encoded by the LGALS3 gene located on human chromosome 14q22.3. The protein contains a carbohydrate recognition domain that binds beta-galactoside residues on glycoproteins and an N-terminal domain enabling oligomerization. This structure allows Galectin-3 to form lattices at the cell surface, influencing receptor clustering and signaling. It is expressed in epithelial cells, macrophages, fibroblasts, and endothelial cells, and can be secreted via a non-classical pathway into the extracellular space. Once outside the cell, it modulates adhesion and migration, contributing to wound healing and fibrosis.
The LGALS3 antibody is particularly useful for detecting Galectin-3 expression in tumor and fibrotic tissues. Western blot typically shows an ~26 kilodalton band, while immunostaining reveals cytoplasmic, nuclear, or membrane-associated localization depending on cellular context. Overexpression of Galectin-3 is associated with aggressive tumor phenotypes, including resistance to apoptosis and enhanced metastasis. Mechanistically, Galectin-3 interacts with cell-surface receptors such as integrins and growth factor receptors, amplifying signaling through the phosphoinositide 3-kinase/AKT and transforming growth factor beta (TGF-beta) pathways. This dual role in intracellular survival signaling and extracellular communication underscores its complexity as a therapeutic target.
Beyond oncology, Galectin-3 contributes to fibrotic progression in the heart, kidneys, and liver. It promotes fibroblast activation, collagen deposition, and tissue stiffening, leading to organ dysfunction. Elevated plasma Galectin-3 levels are now used clinically as a prognostic biomarker for heart failure. In immune regulation, Galectin-3 modulates macrophage polarization, promotes neutrophil adhesion, and suppresses T-cell receptor signaling, linking innate and adaptive immune responses.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
