| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human LGALS3BP recombinant protein (Position: H115-K508) was used as the immunogen for the LGALS3BP antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
LGALS3BP Antibody / Galectin-3-binding protein is a anti-LGALS3BP Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow cytometry (FACS), ELISA with listed reactivity in Human, Rat.
Key elements and design rationale
- Target: LGALS3BP
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, ICC, IF, FACS, ELISA
Biological background
Functionally, LGALS3BP antibody identifies a 585-amino-acid secreted protein characterized by a heavily glycosylated scavenger receptor cysteine-rich (SRCR)-like domain. LGALS3BP interacts with galectins, integrins, and extracellular matrix components such as fibronectin and laminin to facilitate cell adhesion and migration. It modulates immune signaling by promoting cytokine release, dendritic cell maturation, and macrophage activation. In the tumor microenvironment, LGALS3BP enhances metastatic potential by regulating cell-cell interactions and immune evasion mechanisms.
The LGALS3BP gene is located on chromosome 17q25.3 and is expressed in immune cells, epithelial tissues, and cancerous lesions. Its secretion increases in inflammatory conditions, viral infections, and malignancies, where it serves as a biomarker of immune activation and tumor progression.
Pathologically, elevated LGALS3BP levels have been associated with breast, prostate, and lung cancers, as well as HIV infection and autoimmune diseases. In cancer, high expression correlates with increased invasiveness and poor prognosis due to its roles in matrix remodeling and immune suppression. In infectious diseases, LGALS3BP can act as a host restriction factor that limits viral replication. Research using LGALS3BP antibody supports studies in cancer biology, immunology, and extracellular matrix signaling.
LGALS3BP antibody is validated for western blotting, immunohistochemistry, and ELISA to detect secreted glycoproteins.
Structurally, Galectin-3-binding protein forms disulfide-linked oligomers and contains multiple N-linked glycosylation sites critical for stability and receptor binding. Its SRCR-like domain mediates carbohydrate and protein interactions that underpin its diverse functional roles. This antibody enables analysis of LGALS3BP's involvement in cell communication, matrix adhesion, and immune regulation.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
