| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human LGALS7 recombinant protein (Position: M1-F136) was used as the immunogen for the LGALS7/7B antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
LGALS7/7B Antibody / Galectin 7 is a anti-7B Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), ELISA with listed reactivity in Human, Mouse, Rat.
Key elements and design rationale
- Target: 7B
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, ELISA
Biological background
Galectin-7 is expressed abundantly in stratified epithelia, including the skin, cornea, and mucosal linings. It plays an important role in maintaining epithelial homeostasis by regulating keratinocyte differentiation and apoptosis. Upon tissue injury or stress, Galectin-7 is upregulated and released into the extracellular matrix, where it modulates cell-cell adhesion and migration during wound healing. It binds to extracellular glycoproteins such as integrins and laminins, mediating epithelial remodeling and cell communication. In keratinocytes, Galectin-7 localization shifts between the cytoplasm and nucleus depending on differentiation and stress conditions.
At the cellular level, Galectin-7 interacts with intracellular proteins such as Bcl-2, promoting mitochondrial apoptosis under proapoptotic stimuli. It also influences p53-dependent transcriptional regulation of cell death genes. Through these pathways, Galectin-7 acts as a proapoptotic factor in damaged epithelial cells while promoting tissue regeneration. Overexpression of Galectin-7 enhances apoptosis and accelerates wound closure, whereas its loss impairs epithelial renewal and barrier integrity.
The LGALS7/7B antibody is widely used in epithelial biology, dermatology, and oncology research to study differentiation, apoptosis, and cell adhesion. Western blot analysis identifies a 15 kilodalton band corresponding to Galectin-7, and immunohistochemistry reveals strong cytoplasmic and nuclear staining in epithelial layers. This antibody enables analysis of Galectin-7 distribution in normal and neoplastic tissues, supporting studies of epithelial morphogenesis and repair.
In cancer, Galectin-7 shows context-dependent functions—it can suppress tumor progression in certain epithelial cancers by promoting apoptosis but can also enhance metastasis in others by regulating cell migration and extracellular matrix interactions. Altered expression has been reported in breast, cervical, and gastric cancers. The LGALS7/7B antibody is a critical reagent for understanding how glycan-binding proteins contribute to cancer cell plasticity, metastasis, and immune regulation.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
