| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human LGR5 recombinant protein (Position: R26-L907) was used as the immunogen for the LGR5 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
LGR5 Antibody / Leucine-rich repeat-containing G-protein coupled receptor 5 is a anti-LGR5 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat. Reported localization: Golgi, cell membrane.
Key elements and design rationale
- Target: LGR5
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, IHC, FACS, ELISA
Biological background
LGR5 is encoded by the LGR5 gene on human chromosome 12q21.1. The receptor is approximately 907 amino acids long, with seven transmembrane domains typical of GPCRs and a large extracellular region containing leucine-rich repeats that mediate ligand binding. Upon R-spondin binding, LGR5 interacts with Frizzled and LRP5/6 receptors, amplifying Wnt signaling to sustain stem-cell proliferation.
The LGR5 antibody detects a 100-120 kilodalton protein by western blot and exhibits membrane and cytoplasmic localization in proliferative epithelial zones. In the intestine, LGR5 marks crypt base columnar cells capable of regenerating the entire epithelium. In the hair follicle, it labels bulge stem cells responsible for hair cycle renewal.
LGR5 expression is tightly regulated by Wnt activity and is a key marker for cancer stem cells in colorectal and gastric carcinomas. Overexpression promotes tumor initiation and therapy resistance, while knockdown suppresses proliferation and restores differentiation. The receptor modulates beta-catenin stabilization, transcription of target genes, and maintenance of undifferentiated states.
Because of its role in tissue regeneration and cancer stemness, LGR5 is an important biomarker for stem-cell tracking and targeted therapy development.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
