| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human DNA Ligase IV/LIG4 recombinant protein (Position: M1-K876) was used as the immunogen for the LIG4 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
LIG4 Antibody / DNA Ligase 4 is a anti-LIG4 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat. Reported localization: Nuclear.
Key elements and design rationale
- Target: LIG4
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, ICC, IF, FACS, ELISA
Biological background
Functionally, LIG4 antibody identifies a 911-amino-acid ATP-dependent DNA ligase that seals nicks in double-stranded DNA by catalyzing phosphodiester bond formation. LIG4 operates in complex with XRCC4 and XLF/Cernunnos to align DNA ends and complete repair during NHEJ. It also plays a critical role in V(D)J recombination in lymphocytes, supporting immune diversity.
The LIG4 gene is located on chromosome 13q33.3 and is ubiquitously expressed in proliferating tissues. Its expression is highest in immune cells, where DNA recombination occurs frequently. Proper function of LIG4 ensures efficient DNA repair and prevents chromosomal translocations and mutagenesis.
Pathologically, mutations in LIG4 cause Ligase IV syndrome, characterized by microcephaly, developmental delay, immunodeficiency, and radiosensitivity. Deficiency in LIG4 impairs DNA double-strand break repair, leading to genomic instability and increased cancer susceptibility. Research using LIG4 antibody aids in studies of DNA repair, recombination, and genomic maintenance.
LIG4 antibody is validated for western blotting, immunohistochemistry, and immunofluorescence to detect ligase enzymes in DNA damage response pathways.
Structurally, DNA ligase 4 contains an N-terminal DNA-binding domain, an adenylation domain that binds AMP, and a C-terminal XRCC4-interacting region necessary for NHEJ complex formation. This antibody enables detailed analysis of LIG4's enzymatic activity in genome integrity and repair pathways.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
