| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human LINGO2 recombinant protein (Position: C28-R513) was used as the immunogen for the LINGO2 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
LINGO2 Antibody / Leucine-rich repeat and immunoglobulin-like domain-containing protein 2 is a anti-LINGO2 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), ELISA with listed reactivity in Human, Mouse, Rat.
Key elements and design rationale
- Target: LINGO2
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, ELISA
Biological background
Functionally, LINGO2 antibody identifies a 619-amino-acid membrane protein containing multiple leucine-rich repeats (LRRs) and an immunoglobulin-like domain. LINGO2 localizes primarily to neurons and glial cells, where it regulates axon regeneration and myelination processes. It functions as a negative regulator of neurite outgrowth and participates in receptor signaling complexes involving Nogo receptors (NgR) and co-receptors such as p75NTR and LINGO1.
The LINGO2 gene is located on chromosome 9q21.1 and encodes a protein expressed in brain regions including the cerebral cortex, cerebellum, and hippocampus. LINGO2 contributes to synaptic organization and neural circuit refinement, influencing learning and sensory processing. Beyond the central nervous system, it is expressed in the gastrointestinal tract and epithelial tissues, suggesting broader roles in cell communication and barrier integrity.
Genetic studies have linked variants in LINGO2 to neurological and metabolic disorders, including essential tremor, Parkinson's disease, and obesity. These associations suggest that LINGO2 modulates neurotrophic signaling and energy regulation. Its expression pattern overlaps with key neuronal regulators involved in synaptic maintenance, highlighting its importance in neuronal homeostasis and repair.
LINGO2 antibody is widely used in neurobiology, developmental biology, and receptor signaling research. It is suitable for immunohistochemistry, immunofluorescence, and western blotting to detect LINGO2 expression in neural tissues. This antibody supports studies of axon regeneration, neuronal receptor complexes, and synaptic plasticity. In translational research, LINGO2 is investigated as a potential target for neurodegenerative and metabolic diseases.
Structurally, LINGO2 features an extracellular LRR domain forming a horseshoe-like solenoid shape that mediates protein-protein interactions, and a single-pass transmembrane domain anchoring it to the plasma membrane.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
