| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human LMBRD1 recombinant protein (Position: D67-A540) was used as the immunogen for the LMBRD1 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
LMBRD1 Antibody / Lysosomal cobalamin transport escort protein 1 is a anti-LMBRD1 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Flow cytometry (FACS), ELISA with listed reactivity in Human.
Key elements and design rationale
- Target: LMBRD1
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, FACS, ELISA
Biological background
Functionally, LMBRD1 antibody identifies a 540-amino-acid transmembrane protein localized to the lysosomal membrane. LMBRD1 interacts with the lysosomal cobalamin transporter ABCD4 to mediate the transfer of cobalamin from endocytic vesicles to the cytosol. This process is crucial for the formation of methylcobalamin and adenosylcobalamin, cofactors required for methionine synthase and methylmalonyl-CoA mutase activity.
The LMBRD1 gene is located on chromosome 6q13 and is expressed in liver, kidney, and other metabolically active tissues. Its function links lysosomal transport to one-carbon metabolism and mitochondrial energy homeostasis. Proper LMBRD1 activity ensures efficient cobalamin utilization and cellular methylation balance.
Pathologically, mutations in LMBRD1 cause cobalamin deficiency type F (cblF), a metabolic disorder characterized by methylmalonic acidemia and homocystinuria. Defects in LMBRD1 disrupt lysosomal cobalamin release, leading to systemic metabolic dysfunction. Research using LMBRD1 antibody supports studies in lysosomal biology, vitamin metabolism, and inherited metabolic diseases.
LMBRD1 antibody is validated for western blotting, immunofluorescence, and immunohistochemistry to detect lysosomal transport proteins.
Structurally, Lysosomal cobalamin transport escort protein 1 contains multiple transmembrane helices and a cytosolic domain that interacts with ABCD4. This antibody aids in exploring LMBRD1's mechanistic role in cobalamin metabolism and cellular nutrient transport.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
