| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human LMNTD2 recombinant protein (Position: E155-A634) was used as the immunogen for the LMNTD2 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
LMNTD2 Antibody / Lamin tail domain-containing protein 2 is a anti-LMNTD2 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat.
Key elements and design rationale
- Target: LMNTD2
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, FACS, ELISA
Biological background
Lamina-associated proteins link chromatin to the inner nuclear membrane and regulate nuclear stiffness, positioning, and transcriptional activity. Although the precise function of Lamina-associated polypeptide 2-like protein remains under characterization, studies suggest it participates in nuclear envelope remodeling during cell division and differentiation. The LMNTD2 antibody enables researchers to localize the protein in interphase nuclei and monitor its redistribution during mitosis, offering insights into how nuclear membrane components influence cell cycle progression.
LMNTD2 expression has been detected across multiple tissues, including brain, heart, and skeletal muscle, suggesting a structural role in maintaining nuclear integrity in mechanically active cells. The protein may interact with lamins (LMNA, LMNB1) and emerin to stabilize the nuclear lamina. Disruption of such interactions can alter chromatin organization and lead to nuclear shape abnormalities often associated with laminopathies and premature aging syndromes. The LMNTD2 antibody allows investigation of these interactions and potential implications in human disease.
Beyond structural maintenance, Lamina-associated polypeptide 2-like protein may influence transcriptional regulation by tethering specific genomic regions to the nuclear periphery. This positioning can repress or activate gene expression depending on chromatin context. The LMNTD2 antibody is valuable for examining spatial genome organization, nuclear compartmentalization, and mechanosensitive gene regulation, topics of growing interest in cell biology and epigenetics.
The LMNTD2 antibody is effective in immunofluorescence, western blotting, and immunohistochemistry, providing a clear nuclear envelope signal in cell-based assays. Its use helps identify structural changes in disease models, evaluate protein–protein interactions, and assess the impact of mechanical stress on nuclear organization.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
