| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human LPAR5 recombinant protein (Position: A83-L372) was used as the immunogen for the LPAR5 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
LPAR5 Antibody / Lysophosphatidic acid receptor 5 is a anti-LPAR5 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Flow cytometry (FACS), ELISA with listed reactivity in Human.
Key elements and design rationale
- Target: LPAR5
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, FACS, ELISA
Biological background
Functionally, LPAR5 antibody identifies a 353-amino-acid receptor localized to the plasma membrane. LPAR5 activation induces calcium mobilization, cytoskeletal rearrangement, and cytokine release. It contributes to pain sensation, vascular permeability, and immune cell migration. In neuronal cells, LPAR5 influences axonal guidance and neuroinflammation.
The LPAR5 gene is located on chromosome 12p13.31 and is expressed in platelets, lymphocytes, dorsal root ganglia, and intestinal epithelial cells. It plays roles in both physiological and pathological processes such as wound healing, sensory transmission, and inflammation.
Pathologically, dysregulation of LPAR5 signaling has been implicated in neuropathic pain, cancer metastasis, and autoimmune diseases. Inhibition of LPAR5 reduces pain hypersensitivity and inflammatory cytokine production, making it a potential therapeutic target. Research using LPAR5 antibody supports studies in GPCR signaling, lipid biology, and inflammation.
LPAR5 antibody is validated for western blotting, immunohistochemistry, and immunofluorescence to detect LPA receptors.
Structurally, Lysophosphatidic acid receptor 5 contains seven transmembrane helices and intracellular loops that couple to G proteins and scaffolding proteins. This antibody facilitates exploration of LPAR5's signaling mechanisms and receptor-mediated physiological responses.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
