| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human LPXN recombinant protein (Position: E45-H323) was used as the immunogen for the LPXN antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
LPXN Antibody / Leupaxin is a anti-LPXN Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse. Reported localization: Cytoplasm, nucleus, cell membrane.
Key elements and design rationale
- Target: LPXN
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, ICC, IF, FACS, ELISA
Biological background
Structurally, Leupaxin is a 395-amino-acid cytoplasmic protein of approximately 46 kilodaltons that contains four C-terminal LIM domains and an N-terminal LD motif region that binds focal adhesion kinase (FAK), PYK2, and other adhesion regulators. These motifs allow LPXN to function as a scaffold linking structural proteins, tyrosine kinases, and cytoskeletal components at focal adhesions. Its phosphorylation on tyrosine residues by FAK and Src family kinases regulates recruitment to adhesion complexes and determines downstream signaling strength.
The LPXN antibody is widely used in cell biology, immunology, and cancer research to study integrin-mediated signaling, immune cell migration, and cytoskeletal regulation. Western blot analysis detects a 46 kilodalton band corresponding to Leupaxin, while immunofluorescence shows punctate cytoplasmic and peripheral staining consistent with focal adhesion localization. This antibody supports studies examining adhesion turnover, cell spreading, and mechanotransduction in immune and endothelial systems.
Functionally, LPXN modulates adhesion strength and migratory capacity in leukocytes and macrophages by coordinating the FAK/PYK2 and paxillin signaling pathways. It influences podosome formation, phagocytosis, and chemotaxis through its ability to regulate actin polymerization and integrin clustering. Dysregulated LPXN expression has been linked to cancer metastasis, inflammatory disorders, and defective immune cell trafficking. The LPXN antibody provides a reliable tool for evaluating these processes and understanding how adhesion adaptors control immune cell behavior.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
