| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human LRG1 recombinant protein (Position: K41-Q257) was used as the immunogen for the LRG1 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
LRG1 Antibody / Leucine-rich alpha-2-glycoprotein 1 is a anti-LRG1 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), ELISA with listed reactivity in Human, Mouse, Rat.
Key elements and design rationale
- Target: LRG1
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, IHC, ELISA
Biological background
Functionally, LRG1 antibody identifies a 312-amino-acid glycoprotein composed of eight leucine-rich repeats forming a curved solenoid structure. LRG1 modulates endothelial cell behavior by binding endoglin and shifting TGF-beta signaling from the ALK5/SMAD2/3 axis to the ALK1/SMAD1/5/8 pathway, promoting angiogenesis. It also serves as an acute-phase reactant, increasing during infection, inflammation, and tissue injury.
The LRG1 gene is located on chromosome 19p13.3 and is highly expressed in liver, endothelial cells, and neutrophils. LRG1 is secreted into plasma, cerebrospinal fluid, and extracellular matrix, where it influences cell adhesion, migration, and vascular remodeling. Elevated serum LRG1 is a biomarker of cardiovascular and inflammatory diseases.
Pathologically, dysregulation of LRG1 contributes to diabetic retinopathy, fibrosis, tumor angiogenesis, and inflammatory disorders. Overexpression of LRG1 promotes abnormal vessel formation and tissue remodeling, while inhibition can suppress pathological angiogenesis. Research using LRG1 antibody supports studies in vascular biology, immunology, and biomarker discovery.
LRG1 antibody is validated for ELISA, western blotting, and immunohistochemistry to detect secreted and tissue-associated glycoproteins.
Structurally, Leucine-rich alpha-2-glycoprotein 1 adopts a horseshoe-shaped LRR domain that mediates protein-protein interactions with growth factors and receptors. This antibody enables investigation of LRG1's role in vascular signaling and disease progression.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
