| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human LRGUK recombinant protein (Position: E277-E605) was used as the immunogen for the LRGUK antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
LRGUK Antibody / Leucine-rich repeat and guanylate kinase domain-containing protein is a anti-LRGUK Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Flow cytometry (FACS), ELISA with listed reactivity in Human, Rat.
Key elements and design rationale
- Target: LRGUK
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, FACS, ELISA
Biological background
LRGUK is encoded by the LRGUK gene located on human chromosome 7q22.1. The protein is approximately 1,453 amino acids in length and contains multiple leucine-rich repeats and a C-terminal guanylate kinase-like domain that mediates protein-protein interactions. LRGUK localizes to the distal centriole, connecting piece, and midpiece of developing spermatozoa, where it interacts with structural and motor proteins involved in axonemal extension.
The LRGUK antibody detects a 165 kilodalton protein by western blot and shows strong localization in elongating spermatids under immunohistochemistry. LRGUK functions as a molecular linker between microtubules and accessory structures such as the mitochondrial sheath, ensuring proper flagellar alignment and motility. Mutations or knockouts of LRGUK in animal models result in immotile sperm with abnormal tail formation, leading to infertility.
Beyond the testis, low levels of LRGUK expression have been reported in ciliated epithelial tissues, suggesting additional roles in ciliogenesis. By anchoring structural elements within motile cilia, LRGUK contributes to coordinated movement and fluid transport in the respiratory and reproductive tracts.
Because LRGUK serves as a key structural determinant of flagellar integrity and motility, it represents an important marker for studying male reproductive health and cytoskeletal organization.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
