| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human LRRC1 recombinant protein (Position: L120-R497) was used as the immunogen for the LRRC1 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
LRRC1 Antibody / Leucine-rich repeat-containing protein 1 is a anti-LRRC1 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), ELISA with listed reactivity in Human, Mouse, Rat.
Key elements and design rationale
- Target: LRRC1
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, ELISA
Biological background
LRRC1 is encoded by the LRRC1 gene located on human chromosome 6p12.2. The protein is approximately 836 amino acids long and contains multiple leucine-rich repeats and coiled-coil regions that facilitate protein-protein interactions. LRRC1 localizes to cell-cell junctions, where it interacts with polarity regulators such as PAR6 and PARD3, forming complexes that define apical and basolateral membrane domains.
The LRRC1 antibody detects a 95 kilodalton band by western blot and reveals junctional and cortical membrane staining under immunofluorescence. Through its interactions, LRRC1 maintains epithelial barrier integrity and contributes to asymmetric signaling required for tissue organization. In neurons, LRRC1 participates in axon guidance and synapse formation by coordinating polarity-related signaling.
Dysregulation of LRRC1 expression has been associated with tumor progression, particularly in epithelial cancers, where loss of polarity contributes to invasiveness and metastasis. LRRC1 also influences cell migration by regulating cytoskeletal rearrangement and adhesion complex turnover. Studies indicate LRRC1 interacts with small GTPases, linking it to actin dynamics and vesicle trafficking.
Because LRRC1 anchors polarity proteins and signaling regulators, it serves as a critical structural determinant of epithelial and neuronal architecture.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
