| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human LSM11 recombinant protein (Position: A17-Q360) was used as the immunogen for the LSM11 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
LSM11 Antibody / U7 snRNA-associated Sm-like protein LSm11 is a anti-LSM11 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), ELISA with listed reactivity in Human, Rat.
Key elements and design rationale
- Target: LSM11
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, ELISA
Biological background
Functionally, LSM11 antibody identifies a 383-amino-acid nuclear protein that partners with LSM10 and other U7 snRNP components to direct histone mRNA 3'-end cleavage. Unlike polyadenylated transcripts, replication-dependent histone mRNAs end in a conserved stem-loop followed by a purine-rich histone downstream element (HDE). The LSM11-containing U7 snRNP recognizes this sequence and recruits the histone cleavage complex to generate mature histone mRNA. This process ensures tight coupling of histone production to DNA replication during S phase.
The LSM11 gene is located on chromosome 5q35.3 and encodes a nuclear-localized protein expressed in proliferating cells, where histone synthesis is active. LSM11 contains Sm motifs that mediate RNA binding and protein-protein interactions essential for snRNP assembly. It directly interacts with FLASH (FLICE-associated huge protein) and the histone cleavage complex subunits to coordinate endonucleolytic processing of histone pre-mRNAs.
Beyond its canonical role in histone RNA processing, LSM11 contributes to transcriptional regulation and genome stability. It has been implicated in the activation of p53-dependent apoptosis under replication stress conditions through the U7 snRNP-FLASH pathway. By modulating histone synthesis, LSM11 indirectly affects chromatin assembly and DNA packaging during cell division. Perturbation of LSM11 expression leads to cell cycle arrest, defective chromatin condensation, and genomic instability.
LSM11 antibody is widely used in molecular biology, RNA processing, and cell cycle research. It is suitable for immunoblotting, immunofluorescence, and RNA-protein interaction studies to examine LSM11 localization and function. This antibody supports research into histone mRNA metabolism, snRNP assembly, and nuclear RNA processing mechanisms. In cancer and developmental studies, LSM11 detection aids in investigating how histone synthesis regulation impacts proliferation and differentiation.
Structurally, LSM11 shares the characteristic Sm-fold found in RNA-binding LSM proteins, forming ring-like complexes that interact with U7 snRNA. Its N-terminal region is important for FLASH recruitment, while the C-terminal domain stabilizes the U7 snRNP.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
