| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human LSM14A recombinant protein (Position: F105-R404) was used as the immunogen for the LSM14A antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
LSM14A Antibody / RAP55 / AlphaSNBP is a anti-LSM14A Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunoprecipitation (IP), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow cytometry (FACS), ELISA with listed reactivity in Human. Reported localization: Cytoplasm (P-body, stress granule).
Key elements and design rationale
- Target: LSM14A
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, IP, ICC, IF, FACS, ELISA
Biological background
LSM14A is encoded by the LSM14A gene located on human chromosome 19p13.2. The protein is approximately 46 kilodaltons and contains an Sm-like domain that mediates RNA binding and protein-protein interactions. It also includes a C-terminal FDF motif required for association with DDX6 helicase, a central regulator of mRNA decapping and translational repression. LSM14A localizes to discrete cytoplasmic foci corresponding to P-bodies, which dynamically assemble and disassemble in response to stress and cellular signaling. Through its activity, LSM14A influences mRNA stability and translation during differentiation and immune activation.
The LSM14A antibody reveals strong cytoplasmic punctate staining under conditions that promote P-body formation, such as serum starvation or oxidative stress. Western blot analysis typically detects a single band near 51 kilodaltons. Functional studies demonstrate that loss of LSM14A impairs mRNA decay, leading to prolonged expression of transiently expressed transcripts. Moreover, LSM14A participates in innate immune responses by regulating the stability of interferon-related mRNAs, linking RNA metabolism to antiviral defense mechanisms.
Recent research indicates that LSM14A interacts with other RNA granule components, including GW182 and AGO2, bridging mRNA silencing and decay pathways. It also contributes to stress granule dynamics and may influence autophagy signaling through mRNA control. Dysregulation of LSM14A expression has been observed in certain cancers and neurodegenerative diseases, highlighting its importance in cellular stress adaptation.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
