| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human LSP1 recombinant protein (Position: A21-P339) was used as the immunogen for the LSP1 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
LSP1 Antibody / Lymphocyte-specific protein 1 is a anti-LSP1 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat.
Key elements and design rationale
- Target: LSP1
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, FACS, ELISA
Biological background
LSP1 modulates actin cytoskeleton dynamics by linking signaling pathways to filament organization. It participates in neutrophil chemotaxis, macrophage spreading, and lymphocyte trafficking through integrin and chemokine receptor signaling. In endothelial cells, LSP1 promotes actin rearrangement necessary for wound closure and angiogenic migration. It also regulates the stability of cell-cell junctions during inflammation, contributing to vascular permeability control.
The LSP1 antibody is widely used in immunology, vascular biology, and cytoskeletal research to detect Lymphocyte-specific protein 1 expression and function. Western blot analysis identifies a 60 kilodalton band corresponding to LSP1, while immunofluorescence demonstrates filamentous cytoplasmic staining that colocalizes with actin. In immune tissues, LSP1 staining highlights lymphocytes, macrophages, and endothelial cells at sites of inflammation, making it a valuable marker of cell motility and immune activation.
Aberrant expression or phosphorylation of LSP1 has been linked to chronic inflammatory diseases, autoimmune disorders, and tumor metastasis. It functions as a regulator of leukocyte extravasation and tissue infiltration. The LSP1 antibody supports studies of cytoskeletal regulation, immune cell dynamics, and endothelial remodeling.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
