| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human LTBR recombinant protein (Position: Q31-Q404) was used as the immunogen for the LTBR antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
LTBR Antibody / Lymphotoxin-beta receptor is a anti-LTBR Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), ELISA with listed reactivity in Human.
Key elements and design rationale
- Target: LTBR
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, ELISA
Biological background
Functionally, LTBR antibody identifies a 435-amino-acid type I transmembrane receptor expressed on epithelial cells, stromal cells, and endothelial cells but absent from most hematopoietic cells. Upon ligand binding, LTBR triggers activation of NF-kappaB, JNK, and MAPK signaling pathways, leading to expression of cytokines, chemokines, and adhesion molecules. These signals promote lymphoid organogenesis, maintenance of high endothelial venules, and antiviral defense. LTBR signaling also induces controlled apoptosis during immune regulation and tissue remodeling.
The LTBR gene is located on chromosome 12p13.31 and is expressed in lymphoid tissues, intestinal epithelium, and vascular endothelium. It plays a central role in secondary lymphoid organ development, including the formation of lymph nodes and Peyer�s patches, as well as in maintaining immune compartmentalization within mucosal barriers.
Pathologically, dysregulation of LTBR contributes to autoimmune diseases, chronic inflammation, and tumor immunity. Hyperactivation promotes lymphoid neogenesis and ectopic germinal center formation in autoimmune conditions such as rheumatoid arthritis and multiple sclerosis, whereas impaired signaling weakens antiviral responses. In cancer, LTBR signaling can either promote tumor cell survival or enhance immune-mediated tumor clearance depending on context. Research using LTBR antibody supports studies in immunology, inflammation, and cancer biology.
LTBR antibody is validated for flow cytometry, western blotting, and immunohistochemistry to detect TNF receptor family proteins.
Structurally, Lymphotoxin-beta receptor contains four extracellular cysteine-rich domains for ligand binding and a cytoplasmic tail with TRAF-binding motifs that mediate signal transduction. Its modular design allows recruitment of adaptor proteins that determine specific pathway activation. This antibody facilitates detailed analysis of LTBR's roles in cytokine signaling, immune structure formation, and tissue homeostasis.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
