| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human LYAR recombinant protein (Position: M1-K379) was used as the immunogen for the LYAR antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
LYAR Antibody / Cell growth-regulating nucleolar protein is a anti-LYAR Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), ELISA with listed reactivity in Human, Mouse, Rat. Reported localization: Nucleolus.
Key elements and design rationale
- Target: LYAR
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, ICC, IF, IP, ELISA
Biological background
Cell growth-regulating nucleolar protein contains a C2HC-type zinc finger motif and a nuclear localization signal, directing it to the nucleolus where ribosome synthesis occurs. It participates in the early stages of 60S ribosomal subunit formation by binding pre-rRNA and ribonucleoprotein complexes. The LYAR antibody enables detection of this nucleolar marker, supporting studies into the molecular events that control ribosome production and cellular growth rates. Its expression correlates with proliferative activity in rapidly dividing cells.
LYAR has emerged as a multifunctional protein involved in transcriptional regulation and cell cycle progression. It interacts with nucleolin and other nucleolar proteins to coordinate ribosomal RNA processing. Beyond ribosome synthesis, Cell growth-regulating nucleolar protein influences p53 signaling and apoptosis by modulating nucleolar stress responses. The LYAR antibody supports analysis of these pathways, helping define how nucleolar integrity links to cell cycle checkpoints and stress-induced growth arrest.
Overexpression of LYAR has been observed in several cancers, including colorectal, prostate, and hepatocellular carcinoma. It promotes cell proliferation and survival by enhancing rRNA synthesis and suppressing apoptosis. The LYAR antibody enables quantitative assessment of its expression in tumor tissues and cell models, aiding investigation into oncogenic mechanisms involving ribosome biogenesis. In contrast, depletion of LYAR impairs ribosomal maturation and reduces cell growth, underscoring its importance for proliferation control.
The LYAR antibody performs effectively in western blotting, immunofluorescence, and immunohistochemistry, showing distinct nucleolar staining consistent with its localization.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
