| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | A synthesized peptide derived from human Lyn protein was used as the immunogen for the LYN antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
LYN Antibody / Tyrosine-protein kinase Lyn is a anti-LYN Rabbit antibody Recombinant Rabbit Monoclonal clone 32L39 supplied in Liquid format. Recommended for workflows such as Western blot (WB) with listed reactivity in Human, Mouse, Rat.
Key elements and design rationale
- Target: LYN
- Antibody details: Rabbit, Recombinant Rabbit Monoclonal, clone 32L39, isotype Rabbit IgG
- Format: Liquid
- Applications (as listed): WB
Biological background
Tyrosine-protein kinase Lyn functions downstream of antigen and cytokine receptors, phosphorylating immunoreceptor tyrosine-based activation and inhibition motifs. Research using LYN antibody has shown that it can both activate and inhibit signaling depending on context, making it a key modulator of immune responses. Lyn phosphorylates substrates such as SYK, PI3K, and SHP phosphatases, coordinating positive and negative feedback loops. This dual regulatory role highlights its importance in immune tolerance and activation balance.
Studies with LYN antibody have revealed its role in B cell receptor signaling. Tyrosine-protein kinase Lyn initiates signaling by phosphorylating immunoreceptor tyrosine-based activation motifs, recruiting downstream effectors that drive calcium flux, gene transcription, and survival. At the same time, it phosphorylates inhibitory motifs that recruit phosphatases to limit signaling intensity. This balance prevents inappropriate activation while allowing robust responses to pathogens.
Dysregulation of Lyn contributes to disease. Research using LYN antibody has demonstrated that overactive Lyn signaling is implicated in chronic myeloid leukemia and B cell malignancies, where it enhances survival and proliferation. Conversely, deficiency or loss of Lyn function contributes to autoimmune disorders by impairing inhibitory signaling. These findings establish Lyn as a therapeutic target in both cancer and autoimmunity.
Lyn also participates in platelet activation, mast cell degranulation, and macrophage responses, emphasizing its broad roles in hematopoietic physiology. Beyond immune function, Lyn contributes to epithelial and neuronal signaling, expanding its relevance to non-hematopoietic systems. These functions highlight the versatility of Tyrosine-protein kinase Lyn as a signaling hub.
LYN antibody is widely used in western blotting, immunohistochemistry, and immunoprecipitation. Western blotting detects total protein levels and phosphorylation states, immunohistochemistry reveals expression in lymphoid organs, and immunoprecipitation enables mapping of protein complexes. These applications make LYN antibody essential for signaling research.
By supplying validated LYN antibody reagents,
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Monoclonal antibodies provide a defined epitope recognition profile that can support consistent comparisons across experiments.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
