| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human LYRM4 recombinant protein (Position: M1-T91) was used as the immunogen for the LYRM4 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
LYRM4 Antibody / LYR motif-containing protein 4 is a anti-LYRM4 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), ELISA with listed reactivity in Human, Mouse, Rat.
Key elements and design rationale
- Target: LYRM4
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, ELISA
Biological background
LYRM4 is encoded by the LYRM4 gene located on human chromosome 6p25.1. The protein is approximately 108 amino acids long and contains a conserved LYR motif (Leucine-Tyrosine-Arginine) that mediates protein-protein interactions within mitochondrial assembly complexes. LYRM4 localizes to the mitochondrial matrix, where it forms a complex with NFS1, ISD11, and ACP (acyl carrier protein) to generate Fe-S clusters required for electron transport chain enzymes and metabolic enzymes such as aconitase and succinate dehydrogenase.
The LYRM4 antibody detects a 12 kilodalton band by western blot and shows mitochondrial matrix localization under immunofluorescence. LYRM4 is indispensable for mitochondrial biogenesis and redox balance. Its loss leads to impaired respiratory chain function, reduced ATP generation, and increased oxidative stress. Inherited mutations in LYRM4 cause mitochondrial disorders characterized by lactic acidosis, muscle weakness, and neurodegeneration.
Beyond its structural role in Fe-S cluster biogenesis, LYRM4 participates in metabolic adaptation under hypoxia and nutrient deprivation, regulating Fe-S cluster allocation between metabolic enzymes. It also influences lipoic acid synthesis and coenzyme Q biosynthesis through shared pathways in mitochondrial metabolism. Dysregulation of LYRM4 has been associated with oxidative damage and mitochondrial DNA instability in various pathologies, including Parkinson's disease and metabolic syndrome.
Because LYRM4 integrates iron-sulfur metabolism with mitochondrial energy production, it serves as a fundamental component of cellular respiration and redox regulation.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
