| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human LZTS2 recombinant protein (Position: M1-I669) was used as the immunogen for the LZTS2 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
LZTS2 Antibody / Leucine zipper putative tumor suppressor 2 is a anti-LZTS2 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat.
Key elements and design rationale
- Target: LZTS2
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, ICC, IF, FACS, ELISA
Biological background
Leucine zipper putative tumor suppressor 2 contains multiple coiled-coil and leucine zipper motifs that facilitate protein-protein interactions and localization to microtubules and centrosomes. During mitosis, it associates with the spindle apparatus and contributes to chromosome segregation fidelity. The LZTS2 antibody supports localization studies that reveal its role in maintaining mitotic stability and preventing aneuploidy. Loss of LZTS2 disrupts spindle organization and cell division, leading to chromosomal instability that can promote tumorigenesis.
As a regulator of Wnt/beta-catenin signaling, Leucine zipper putative tumor suppressor 2 binds beta-catenin and inhibits its nuclear translocation, thereby attenuating transcription of Wnt target genes involved in proliferation. The LZTS2 antibody enables detection of expression changes in response to Wnt pathway activation or tumor progression. Reduced levels of LZTS2 have been documented in prostate, colorectal, and lung cancers, correlating with enhanced beta-catenin activity and increased malignancy.
Beyond cancer biology, Leucine zipper putative tumor suppressor 2 contributes to neural development and cytoskeletal regulation in neurons. It stabilizes microtubule structures and promotes axonal outgrowth, suggesting functions in cellular differentiation and morphogenesis. The LZTS2 antibody supports studies into these roles, providing a means to evaluate expression across developing tissues and in response to neurotrophic factors.
The LZTS2 antibody performs effectively in western blotting, immunofluorescence, and immunohistochemistry, producing characteristic cytoplasmic and perinuclear staining consistent with microtubule association.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
