| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human MAGI2 recombinant protein (Position: Q40-P1140) was used as the immunogen for the MAGI2 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
MAGI2 Antibody / Membrane-associated guanylate kinase inverted 2 is a anti-MAGI2 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat.
Key elements and design rationale
- Target: MAGI2
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, FACS, ELISA
Biological background
MAGI2 is encoded by the MAGI2 gene on human chromosome 7q21.11. The protein is approximately 150 kilodaltons and composed of multiple PDZ, WW, and guanylate kinase-like domains that mediate protein-protein interactions. MAGI2 localizes to postsynaptic densities in neurons and to tight junctions in epithelial cells, where it scaffolds transmembrane receptors such as PTEN, NMDA receptor subunits, and beta-catenin.
MAGI2 regulates synaptic plasticity by stabilizing glutamate receptor complexes and maintaining postsynaptic signaling strength. It also anchors PTEN to the plasma membrane, contributing to phosphoinositide signaling and tumor suppression.
Defects or deletions in MAGI2 are associated with neurological disorders including autism spectrum disorder and epilepsy, as well as cancers involving disrupted cell polarity. In epithelial cells, MAGI2 supports junctional integrity and signal compartmentalization, preventing uncontrolled growth and maintaining barrier function.
As a multifunctional scaffolding protein, MAGI2 integrates membrane signaling with structural organization.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
