| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human MALSU1 recombinant protein (Position: A30-E234) was used as the immunogen for the MALSU1 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
MALSU1 Antibody / Mitochondrial assembly of ribosomal large subunit protein 1 is a anti-MALSU1 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as ELISA, Flow cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (IHC), Western blot (WB) with listed reactivity in Human. Reported localization: Cytoplasm (Mitochondria).
Key elements and design rationale
- Target: MALSU1
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): ELISA, FACS, IF, IHC, WB
Biological background
Mitochondrial assembly of ribosomal large subunit protein 1 interacts with mitochondrial ribosomal proteins and assembly chaperones such as GTPBP10 and L0R8F8. It forms part of a transient anti-association module that maintains 39S subunits in an assembly-competent state. The MALSU1 antibody supports localization and co-immunoprecipitation studies that clarify its role in controlling mitoribosome biogenesis. Loss of MALSU1 results in defective translation and respiratory-chain deficiency, highlighting its importance for mitochondrial function.
Because mitochondrial protein synthesis is required for oxidative phosphorylation, disruption of MALSU1 impairs ATP production and promotes metabolic dysfunction. The MALSU1 antibody supports functional analyses in models of mitochondrial disease, helping quantify expression and assembly defects. By stabilizing immature ribosomal subunits, MALSU1 safeguards the fidelity of mitochondrial translation, contributing to overall cellular energy homeostasis.
Beyond mitochondrial biogenesis, Mitochondrial assembly of ribosomal large subunit protein 1 has been implicated in apoptosis regulation and stress adaptation. Its expression increases during mitochondrial stress to support recovery of ribosome integrity. The MALSU1 antibody enables investigation of these adaptive responses, providing insight into how mitochondrial translation is coordinated with quality-control pathways.
The MALSU1 antibody performs effectively in western blotting, immunofluorescence, and immunohistochemistry, producing punctate mitochondrial staining consistent with its subcellular localization.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
