| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human MAML1 recombinant protein (Position: M1-K998) was used as the immunogen for the MAML1 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
MAML1 Antibody / Mastermind-like protein 1 is a anti-MAML1 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunofluorescence (IF), Flow cytometry (FACS), ELISA with listed reactivity in Human.
Key elements and design rationale
- Target: MAML1
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, IF, FACS, ELISA
Biological background
MAML1 contains an N-terminal basic domain responsible for binding NICD and CSL, a central acidic transcriptional activation domain, and a C-terminal domain that mediates interactions with additional transcription factors and coactivators such as p300/CBP. Through these interactions, MAML1 promotes transcription of Notch target genes including HES1, HEY1, and MYC, which are involved in cell differentiation and proliferation. Because Notch signaling is crucial for tissue development and homeostasis, MAML1 serves as a master regulator of developmental pathways across multiple cell types, including neural, hematopoietic, and epithelial lineages.
The MAML1 antibody is widely used in molecular biology, developmental biology, and cancer research to assess Notch pathway activation and transcriptional regulation. Western blot analysis typically identifies a 115 kilodalton band corresponding to MAML1, while immunofluorescence reveals nuclear localization that overlaps with Notch pathway components. This antibody enables the study of transcriptional coactivation events and gene regulation in Notch-mediated signaling contexts.
Beyond Notch signaling, MAML1 also functions as a coactivator for other transcriptional regulators such as MEF2C, p53, and beta-catenin, broadening its impact on gene networks related to cell growth and apoptosis. Dysregulation of MAML1 has been linked to oncogenic transformation, particularly in leukemia, glioma, and breast cancer, where aberrant Notch signaling drives uncontrolled proliferation. The MAML1 antibody provides a reliable tool for investigating transcriptional complexes and signaling crosstalk mechanisms.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
