| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human MAML3 recombinant protein (Position: H87-P1138) was used as the immunogen for the MAML3 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
MAML3 Antibody / Mastermind-like protein 3 is a anti-MAML3 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), Flow cytometry (FACS), ELISA with listed reactivity in Human. Reported localization: Nuclear speckles.
Key elements and design rationale
- Target: MAML3
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, IHC, IF, ICC/IF, FACS, ELISA
Biological background
Mastermind-like protein 3 acts as a scaffold that stabilizes the Notch-CSL complex on target promoters, recruiting additional coactivators such as p300/CBP to initiate transcription. Its activation leads to expression of Notch target genes, including those involved in stem cell maintenance, neural development, and tumorigenesis. The MAML3 antibody supports studies aimed at elucidating these transcriptional networks by allowing detection and localization of MAML3 in nuclear extracts and tissue sections.
Chromosomal rearrangements involving MAML3 have been implicated in human cancers, notably biphenotypic sinonasal sarcoma and certain neuroendocrine tumors. The resulting fusion proteins, such as PAX3-MAML3, act as potent transcriptional activators driving oncogenic programs. The MAML3 antibody enables researchers to analyze wild-type and fusion variants, supporting investigations into the molecular mechanisms by which Mastermind-like protein 3 contributes to tumor development and aberrant Notch signaling.
Beyond its canonical Notch-related functions, Mastermind-like protein 3 participates in other signaling pathways, including Wnt and Hedgehog, where it modulates transcriptional responses through interactions with diverse transcription factors. The MAML3 antibody aids in identifying these alternative regulatory roles, helping to map MAML3-containing transcriptional complexes in various tissues and developmental stages. Expression analysis indicates enrichment of MAML3 in neural and mesenchymal lineages, consistent with its role in differentiation control.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
