| Field | Specification |
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| Mfr No | |
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| Host | |
| Immunogen | E.coli-derived human MAN2C1 recombinant protein (Position: A176-Q1027) was used as the immunogen for the MAN2C1 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
MAN2C1 Antibody / Mannosidase alpha class 2C member 1 is a anti-MAN2C1 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat. Reported localization: Nuclear.
Key elements and design rationale
- Target: MAN2C1
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, ICC, IF, FACS, ELISA
Biological background
Mannosidase alpha class 2C member 1 is expressed in a wide range of tissues, including liver, kidney, and brain, where it contributes to the breakdown of misfolded glycoproteins following endoplasmic reticulum�associated degradation (ERAD). As improperly folded glycoproteins are retro-translocated to the cytosol for proteasomal degradation, MAN2C1 helps trim mannose residues from liberated oligosaccharides. This function is key for maintaining protein quality control and preventing toxic accumulation of misprocessed glycans. The MAN2C1 antibody is frequently employed in studies focused on carbohydrate metabolism, glycoprotein turnover, and the molecular mechanisms that connect glycan processing with proteostasis.
In addition to its metabolic role, Mannosidase alpha class 2C member 1 has been implicated in cell signaling and tumor biology. Evidence suggests that overexpression of MAN2C1 may suppress PTEN activity and promote oncogenic signaling through the AKT pathway. Conversely, reduced enzyme expression can influence apoptosis sensitivity and cell-cycle regulation. These findings highlight the enzyme's dual role as both a metabolic and signaling regulator. The MAN2C1 antibody allows scientists to quantify these expression changes and assess protein�protein interactions that link glycan metabolism to oncogenesis.
Experimental use of the MAN2C1 antibody includes western blotting for protein detection, immunofluorescence for subcellular localization, and immunohistochemistry for tissue-level expression mapping. The enzyme is mainly cytoplasmic but may associate with endoplasmic reticulum membranes under certain physiological conditions. Researchers utilize the MAN2C1 antibody to characterize these distribution patterns and examine how post-translational modifications affect enzyme stability and activity.
Because glycoprotein metabolism influences immune recognition and cellular signaling, Mannosidase alpha class 2C member 1 has become an attractive research target across immunology, cancer, and metabolic disease. The MAN2C1 antibody provided by
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
