| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human MAP1B recombinant protein (Position: H102-R1849) was used as the immunogen for the MAP1B antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
MAP1B Antibody / Microtubule-associated protein 1B is a anti-MAP1B Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat.
Key elements and design rationale
- Target: MAP1B
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, IHC, IF, FACS, ELISA
Biological background
Functionally, MAP1B antibody identifies a precursor protein of approximately 2,460 amino acids that is proteolytically cleaved into heavy and light chains. These subunits assemble with microtubules and actin filaments, promoting cytoskeletal crosslinking. MAP1B is enriched in developing neurons, where it modulates growth cone dynamics and neurite outgrowth, influencing axonal pathfinding and regeneration.
The MAP1B gene is located on chromosome 5q13.2 and is highly expressed in the central and peripheral nervous systems. MAP1B associates with other neuronal cytoskeletal proteins, such as tau and MAP2, to regulate polymerization and microtubule stability. Phosphorylation of MAP1B by kinases including GSK3 and CDK5 modulates its binding affinity and spatial localization, fine-tuning neuronal polarity and maturation.
Pathologically, aberrant MAP1B expression or phosphorylation is associated with neurodevelopmental and neurodegenerative diseases, including Alzheimer�s and Parkinson�s disease. In tumor cells, MAP1B re-expression correlates with altered cytoskeletal organization and invasiveness. Research using MAP1B antibody supports studies in axonal transport, cytoskeletal remodeling, and neuronal repair mechanisms.
MAP1B antibody is validated for western blotting, immunohistochemistry, and immunofluorescence to detect neuronal microtubule-associated proteins.
Structurally, Microtubule-associated protein 1B contains multiple microtubule-binding domains and an actin-binding region, facilitating cytoskeletal integration. Its dynamic phosphorylation pattern determines its localization and activity during neuron growth and regeneration. This antibody provides a reliable tool to examine MAP1B's regulatory functions in cytoskeletal organization and axonal development.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
