| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human MEK1/MAP2K1 recombinant protein (Position: M1-V393) was used as the immunogen for the MAP2K1 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
MAP2K1 Antibody / Mitogen-activated protein kinase kinase 1 is a anti-MAP2K1 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as ELISA, Flow cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Western blot (WB) with listed reactivity in Human, Mouse, Rat. Reported localization: Cytoplasmic, Nuclear.
Key elements and design rationale
- Target: MAP2K1
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): ELISA, FACS, IF, IHC, ICC, WB
Biological background
MAP2K1 is encoded by the MAP2K1 gene located on human chromosome 15q22.31. The protein is approximately 393 amino acids in length and consists of an N-terminal regulatory region and a C-terminal kinase domain. MAP2K1 belongs to the family of dual-specificity kinases, capable of phosphorylating both threonine and tyrosine residues on its substrates. Activation occurs through phosphorylation by RAF kinases in response to growth factors, hormones, or stress stimuli.
The MAP2K1 antibody detects a 45 kilodalton protein by western blot and reveals cytoplasmic and nuclear localization under immunofluorescence microscopy. In resting cells, MAP2K1 is inactive; upon stimulation of RAS-RAF signaling, it becomes phosphorylated at serine residues 218 and 222, enabling catalytic activation of ERK kinases. This cascade transmits extracellular signals to transcription factors, leading to gene expression changes that drive cell proliferation and differentiation.
Aberrant MAP2K1 activation is implicated in numerous cancers, including melanoma, colorectal, and lung carcinoma, due to constitutive activation of the MAPK pathway. Mutations that enhance MAP2K1 activity or confer resistance to RAF inhibitors are clinically significant in targeted cancer therapy. Beyond oncology, MAP2K1 contributes to cardiac hypertrophy, immune signaling, and neural plasticity.
Because of its central role in MAPK pathway regulation, MAP2K1 serves as a key target for understanding cell signaling and therapeutic intervention.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
