| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | A synthesized peptide derived from human MK3 was used as the immunogen for the MAPKAPK3 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
MAPKAPK3 Antibody / Mitogen activated protein kinase activated protein kinase 3 is a anti-MAPKAPK3 Rabbit antibody Recombinant Rabbit Monoclonal clone 30M15 supplied in Liquid format. Recommended for workflows such as Western blot (WB), Immunoprecipitation (IP) with listed reactivity in Human.
Key elements and design rationale
- Target: MAPKAPK3
- Antibody details: Rabbit, Recombinant Rabbit Monoclonal, clone 30M15, isotype Rabbit IgG
- Format: Liquid
- Applications (as listed): WB, IP
Biological background
Mitogen activated protein kinase activated protein kinase 3 phosphorylates a variety of substrates, including transcription factors, RNA binding proteins, and regulators of actin dynamics. This allows MAPKAPK3 to integrate stress signals with gene expression programs and structural remodeling. Studies with MAPKAPK3 antibody have shown that it participates in the regulation of CREB and ATF1 transcription factors, influencing expression of stress inducible genes. It also phosphorylates the small heat shock protein HSP27, altering cytoskeletal dynamics and cell motility. These diverse downstream targets highlight the broad influence of MAPKAPK3 in cellular adaptation.
Dysregulation of MAPKAPK3 signaling has been implicated in inflammation, cancer, and neurological disorders. Overactivation of p38 MAPK and its downstream kinases contributes to chronic inflammatory states and has been studied in conditions such as rheumatoid arthritis and inflammatory bowel disease. Research with MAPKAPK3 antibody has revealed that altered MAPKAPK3 activity can promote tumorigenesis by enhancing survival pathways and stress resistance in cancer cells. In the nervous system, MAPKAPK3 has been implicated in synaptic plasticity and memory, suggesting that disruption of its activity may contribute to cognitive impairment and neurodegeneration.
MAPKAPK3 antibody is widely applied in western blotting, immunohistochemistry, and kinase assays. Western blotting detects activation and phosphorylation states, while immunohistochemistry reveals tissue specific expression patterns. Kinase assays using immunoprecipitated MAPKAPK3 protein help identify novel substrates and confirm functional activity. These approaches allow comprehensive characterization of MAPKAPK3 signaling in both health and disease.
By providing validated MAPKAPK3 antibody reagents,
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Monoclonal antibodies provide a defined epitope recognition profile that can support consistent comparisons across experiments.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
