| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | A portion of amino acids 4-32 from the human protein was used as the immunogen for this MARCH5 antibody. |
| Isotype | |
| Product Type | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
MARCH5 Antibody / Membrane-associated RING finger protein 5 is an antibody targeting MARCH5, raised in Rabbit for protein detection and localization studies where these specifications are required.
Key elements and design rationale
- Target: MARCH5 (reported localization: Cytoplasm).
- Antibody identity: Polyclonal (rabbit origin); Rabbit Ig.
- Conjugate/label: Unconjugated (affects detection chemistry and multiplex compatibility).
- Format: Purified.
- Species reactivity: Human, Mouse.
- Listed applications: WB, IHC-P (refer to on-page specifications for application-specific guidance).
Biological background
Membrane-associated RING finger protein 5 (MARCH5), also known as MARCHF5, is involved in cellular processes that are essential for maintaining the homeostasis and integrity of the cell. It possesses a RING finger domain that allows it to function as an E3 ubiquitin ligase, mediating the ubiquitination and subsequent degradation of specific target proteins. One of the key functions of MARCH5 is its role in regulating the turnover of mitochondrial proteins. Studies have shown that MARCH5 targets a variety of mitochondrial proteins for ubiquitination, leading to their degradation by the proteasome. This process helps to maintain the quality control of mitochondria and ensures the proper functioning of these vital organelles. Furthermore, MARCH5 has been implicated in various cellular processes, including apoptosis, autophagy, and immune response. By targeting specific proteins for degradation, MARCH5 can regulate the signaling pathways that govern these processes, influencing cell survival, proliferation, and immunity. MARCH5 has also been linked to the development of certain diseases, such as Parkinson's disease and cancer. Dysregulation of MARCH5 expression or activity has been observed in these conditions.
Research relevance and current trends
- Comparative expression profiling across cell types, tissues, or perturbations (e.g., drug treatment, genetic editing, or differentiation).
- Subcellular localization and trafficking studies, including co-localization with pathway markers in microscopy-based assays.
- Integration of protein-level measurements with transcriptomics or proteomics to relate abundance to regulation and phenotype.
Common research applications
- Western blotting: researchers commonly compare relative signal levels across conditions and use appropriate negative/positive controls for interpretation.
- Immunohistochemistry: researchers commonly compare relative signal levels across conditions and use appropriate negative/positive controls for interpretation.
Interpretation should account for antibody-dependent factors such as epitope accessibility, isoforms, and sample preparation differences across workflows.
Notes for experimental interpretation
- Isoforms and PTMs: many targets have multiple isoforms and post-translational modifications that can shift apparent signal or localization; interpret bands/signals accordingly.
- Epitope context: binding can depend on protein conformation and sample processing; region information in the title/immunogen can help anticipate what may be detected.
- Species differences: predicted or validated reactivity may vary by ortholog sequence and sample context; confirm in your model system.
- Control concepts: include negative controls (no-primary/isotype), and where possible genetic controls (KO/KD) or independent antibodies to strengthen conclusions.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
