| Field | Specification |
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| Clonality | |
| Host | |
| Immunogen | A synthetic peptide corresponding to a sequence at the C-terminus of human MARCKS was used as the immunogen for the MARCKS antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
MARCKS Antibody / Myristoylated alanine-rich C-kinase substrate is a anti-MARCKS Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Flow cytometry (FACS) with listed reactivity in Human, Mouse, Rat. Reported localization: Cytoplasm, cell membrane.
Key elements and design rationale
- Target: MARCKS
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, IHC, FACS
Biological background
MARCKS is encoded by the MARCKS gene located on human chromosome 6q22.2. The protein is approximately 32 kilodaltons in size and is characterized by a highly basic effector domain that mediates binding to actin filaments, calmodulin, and acidic phospholipids such as phosphatidylinositol 4,5-bisphosphate (PIP2). N-terminal myristoylation allows MARCKS to anchor reversibly to the plasma membrane. Activation of protein kinase C induces phosphorylation of specific serine residues within the effector domain, causing detachment from the membrane and release of bound PIP2, thus modulating downstream calcium signaling and actin polymerization.
Using the MARCKS antibody, researchers can detect phosphorylated and non-phosphorylated forms of the protein by western blot and immunofluorescence. Western blot typically reveals a single band around 32-35 kilodaltons, while phospho-specific variants of MARCKS migrate slower due to phosphorylation-induced charge differences. Immunostaining shows cortical and membrane localization in unphosphorylated states, transitioning to a diffuse cytoplasmic distribution following stimulation with phorbol esters or calcium ionophores. MARCKS plays critical roles in cell migration, exocytosis, phagocytosis, and neuronal growth cone extension, making it a widely studied effector of signal-dependent cytoskeletal remodeling.
In the nervous system, MARCKS regulates dendritic spine morphology, synaptic vesicle trafficking, and axonal guidance. It is enriched in developing neurons and contributes to long-term potentiation and synaptic plasticity. In non-neuronal tissues, MARCKS is implicated in wound healing, immune cell chemotaxis, and cancer metastasis. Overexpression and dysregulation have been observed in glioblastoma, breast cancer, and inflammatory diseases, highlighting its function as a signaling nexus controlling cell motility and proliferation.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
