| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human MARCO recombinant protein (Position: M1-R489) was used as the immunogen for the MARCO antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
MARCO Antibody / Macrophage receptor with collagenous structure is a anti-MARCO Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Flow cytometry (FACS), ELISA with listed reactivity in Human.
Key elements and design rationale
- Target: MARCO
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, FACS, ELISA
Biological background
As a pattern recognition receptor (PRR), MARCO recognizes both Gram-positive and Gram-negative bacterial components, including lipopolysaccharides, lipoteichoic acids, and bacterial DNA. Through its SRCR domain, MARCO antibody detects the receptor�s role in phagocytosis and immune surveillance. Upon ligand binding, MARCO facilitates pathogen uptake and triggers downstream signaling pathways that regulate inflammation and macrophage activation. It cooperates with other receptors such as TLR2, TLR4, and CD14 to enhance recognition and clearance of pathogens. Functionally, MARCO acts as both a scavenger and adhesion molecule, mediating macrophage binding to microbial surfaces and extracellular matrix components.
MARCO expression is inducible by inflammatory stimuli such as lipopolysaccharide (LPS), interferon gamma, and bacterial infection. In the lung, alveolar macrophages expressing MARCO are essential for clearance of inhaled pathogens and environmental particulates, protecting against infection and tissue damage. Experimental models show that MARCO-deficient mice have impaired clearance of Streptococcus pneumoniae, Listeria monocytogenes, and Mycobacterium tuberculosis, emphasizing its importance in pulmonary immunity. The receptor also binds oxidized lipids and apoptotic cells, participating in tissue remodeling and atherosclerosis prevention.
MARCO is part of the macrophage scavenger receptor family that includes SR-A1 (MSR1) and SCARA5. Among these, MARCO is distinguished by its extended collagenous domain and unique ligand specificity. Structurally, the SRCR domain coordinates divalent metal ions required for ligand interaction and receptor clustering on the cell surface. The receptor forms trimers stabilized by disulfide bonds, allowing it to crosslink and internalize large particles. In addition to macrophages, MARCO can be transiently expressed on dendritic cells and certain epithelial cells under inflammatory conditions.
Disease associations highlight MARCO's dual role in host defense and inflammation. Variants in the MARCO gene have been linked to increased susceptibility to pulmonary tuberculosis and chronic obstructive pulmonary disease (COPD). Dysregulated MARCO expression contributes to granulomatous inflammation and altered immune responses in respiratory disorders. In neuroinflammation, microglial MARCO participates in clearance of amyloid-beta aggregates, suggesting a role in Alzheimer�s disease pathology. Moreover, tumor-associated macrophages expressing MARCO promote immunosuppression in certain cancers, making the receptor a target of emerging cancer immunotherapies aimed at reprogramming macrophage activity.
Immunohistochemical staining using MARCO antibody reveals strong membrane and cytoplasmic localization in splenic red pulp macrophages, alveolar macrophages, and Kupffer cells of the liver. This antibody serves as a key reagent in studying macrophage differentiation, pathogen-host interaction, and innate immune mechanisms. MARCO antibody from
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
