| Field | Specification |
|---|---|
| Mfr No | |
| Product Type | |
| Promoter | |
| Reporter | |
| Selection Marker | Blasticidin, Puromycin |
| Shipping | |
| Species |
Background
The MAF recognition element (MARE) is the DNA sequence bound by the large MAF family of basic leucine zipper transcription factors, which includes MAFA, MAFB, c-MAF, and NRL. These factors bind MARE sequences as homodimers or as heterodimers with other bZIP proteins to regulate cell-type-specific gene expression. Large MAF proteins are important for terminal differentiation of multiple lineages, contributing to processes such as lens development, pancreatic beta cell function, and macrophage and immune cell differentiation. Dysregulated MAF expression is associated with certain cancers, including multiple myeloma. MAF transcription factors are therefore relevant to studies of differentiation, development, and disease.
Product Description & Applications
The MARE Reporter Lentivirus is a transcription factor reporter system that provides a sensitive fluorescent or luminescent readout of MAF bZIP transcription factor activity in human or mouse cells. The construct uses tandem repeats of MAF response elements (T-MARE) upstream of a fluorescent (GFP, RFP, EGFP, d2GFP, mCherry) or luminescent (firefly luciferase) reporter, enabling detection of transcriptional activity of MAF family factors such as c-MAF, MAFB, and related proteins. Antibiotic selection markers (puromycin or blasticidin) support establishment of stable reporter cell lines. The system is used to study MAF-driven transcription, cell differentiation, and the signaling that regulates MAF activity. Supplied as lentiviral particles purified by PEG precipitation and sucrose gradient centrifugation, it efficiently transduces difficult-to-transfect cells, including primary and thawed cells.
About This Product
This reporter lentivirus places a d2GFP, EGFP, Firefly Luc, GFP, mCherry, RFP reporter gene under the control of tandem consensus response elements specific for the MAF Signaling Pathway transcription factor, coupled to a minimal TATA-box promoter and a proprietary upstream enhancer that maximizes signal-to-noise. The constitutively expressed selection marker (Blasticidin, Puromycin) and/or secondary reporter enables stable polyclonal cell line generation and flexible readout by fluorescence microscopy, flow cytometry, or luminometry.
Stable integration via the lentiviral backbone ensures consistent, clonally representative reporter expression in dividing and post-mitotic target cells — including primary T cells, macrophages, organoids, and cryopreserved material — eliminating the variability inherent to transient transfection. The self-inactivating LTR design and third-generation packaging minimize insertional mutagenesis risk and ensure biosafety classification at BSL-2.
Can't find the lentiviral construct you need, or want to adjust key design elements? Contact us to discuss custom LV design and optional add-ons.
Common customization requests
- Insert / payload: replace the gene/sequence, swap to a different isoform, add mutations, or optimize cloning features.
- Expression design: change promoter (e.g., CMV/EF1α/PGK), add enhancers, or adjust regulatory elements.
- Reporters: add/swap GFP/RFP/mCherry/luciferase (single or dual reporters where applicable).
- Selection markers: add/swap puromycin/blasticidin/neomycin or fluorescent selection options.
- Vector format: switch between OE, shRNA, CRISPR (sgRNA/Cas systems), or control vectors (where supported).
Add-ons you can request
- Control viruses: empty vector, non-targeting shRNA, reporter-only controls, or matched backbone controls.
- Packaging / format: concentration options, aliquoting, or custom fill volume for screening workflows.
- Documentation: construct map/sequence confirmation package (as available) and batch documentation.
What to include in your request
- Target cell type/model (cell line or primary cells) and intended readout (reporter, knockdown, OE, etc.)
- Insert sequence (FASTA) or reference ID, plus any required tags/mutations
- Promoter, reporter, and selection marker preferences
- Desired scale and preferred format (aliquots / concentration requests)
Email us at support@biohippo.com or use the Talk to a Scientist request form.
