| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human MARVELD2 recombinant protein (Position: R12-H496) was used as the immunogen for the MARVELD2 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
MARVELD2 Antibody / Tricellulin is a anti-MARVELD2 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Flow cytometry (FACS), ELISA with listed reactivity in Human, Rat. Reported localization: Cell junctions.
Key elements and design rationale
- Target: MARVELD2
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, IHC, FACS, ELISA
Biological background
MARVELD2 is encoded by the MARVELD2 gene located on human chromosome 5q13.1. The protein is approximately 558 amino acids long and contains four transmembrane segments with a cytoplasmic C-terminal tail. It localizes at tricellular tight junctions, distinct from bicellular tight junction proteins such as occludin and claudins. MARVELD2 interacts with angulin family proteins and zonula occludens proteins to anchor the tricellular junction complex to the actin cytoskeleton.
The MARVELD2 antibody detects a 65 kilodalton band by western blot and exhibits strong junctional staining along epithelial borders in immunofluorescence. MARVELD2 regulates diffusion barrier properties by sealing the tricellular junction core, ensuring that epithelial sheets restrict large solute movement. Loss of MARVELD2 disrupts paracellular sealing, resulting in barrier leakage and increased susceptibility to inflammation and infection.
Mutations in MARVELD2 cause autosomal recessive deafness (DFNB49) due to impaired tight junction formation in the cochlea, leading to hair cell degeneration and hearing loss. Altered expression is also associated with inflammatory bowel disease and cancer metastasis, where weakened epithelial integrity promotes tissue invasion and immune infiltration.
As a critical determinant of epithelial sealing and tissue organization, MARVELD2 serves as a biomarker for tight junction function and epithelial polarity.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
