| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human MATK recombinant protein (Position: S18-Q298) was used as the immunogen for the MATK antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
MATK Antibody / Megakaryocyte-associated tyrosine kinase is a anti-MATK Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat. Reported localization: Cytoplasm, Membrane.
Key elements and design rationale
- Target: MATK
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, IHC, ICC, IF, FACS, ELISA
Biological background
Structurally, MATK contains an SH3 domain, an SH2 domain, and a C-terminal catalytic kinase domain, similar to Csk, but it lacks the N-terminal myristoylation signal that targets Csk to membranes. This structural difference restricts MATK mainly to the cytoplasm, where it regulates Src kinases indirectly through adaptor-mediated interactions. MATK belongs to the protein tyrosine kinase family and shares homology with other signaling regulators such as CSK and CHK. Known interacting partners include PAG1, LAT, and LYN, which facilitate recruitment to signaling complexes.
Functionally, MATK phosphorylates inhibitory tyrosine residues within Src family kinases, leading to their inactivation and termination of receptor signaling. In platelets, MATK regulates activation by suppressing FYN and LYN kinase signaling downstream of GPVI and integrins. In immune cells, it modulates T-cell receptor (TCR) and B-cell receptor (BCR) signaling, helping to prevent overactivation and maintain immune tolerance. MATK also participates in neuronal and endothelial cell signaling, contributing to cytoskeletal organization and vascular homeostasis.
MATK plays an important role in preventing hyperactivation of immune responses and maintaining hemostatic balance. Dysregulation of MATK expression or activity has been associated with autoimmune disorders, inflammatory diseases, and malignancies. Reduced MATK expression can result in excessive Src kinase activity, contributing to aberrant cell growth and survival. Conversely, overexpression has been observed in certain leukemias, where it suppresses proliferation signaling. Pathway involvement includes Src kinase signaling, platelet activation, and immune receptor regulation.
The MATK antibody from
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
