| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human Matrilin-4/MATN4 recombinant protein (Position: E191-Q590) was used as the immunogen for the MATN4 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
MATN4 Antibody / Matrilin 4 is a anti-MATN4 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Flow cytometry (FACS), ELISA with listed reactivity in Human.
Key elements and design rationale
- Target: MATN4
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, FACS, ELISA
Biological background
Functionally, MATN4 antibody identifies a 602-amino-acid modular protein containing von Willebrand factor A (vWFA) domains and coiled-coil motifs. MATN4 interacts with matrix components such as aggrecan, COMP, and collagens II and IX, facilitating the formation of filamentous networks that provide tensile strength and elasticity to connective tissues. It plays a structural and regulatory role in cartilage organization and tissue resilience under mechanical load.
The MATN4 gene is located on chromosome 20q13.12 and encodes a protein expressed primarily in skeletal tissues, including cartilage, intervertebral discs, and developing bone. Matrilin-4 functions as an adaptor molecule linking fibrillar collagens to proteoglycan aggregates, stabilizing the extracellular matrix architecture. During development, MATN4 expression is tightly regulated by transcription factors such as SOX9 and RUNX2 that control chondrogenesis and osteogenesis.
In physiology, MATN4 supports cartilage integrity and joint function. Reduced expression or mutations in matrilin family proteins can impair cartilage matrix stability, predisposing to osteoarthritis or connective tissue degeneration. MATN4 also contributes to tissue repair by enhancing chondrocyte adhesion and matrix assembly following injury. In cancer biology, altered MATN4 expression has been reported in breast, gastric, and pancreatic cancers, where it may influence tumor cell adhesion and invasion through modulation of the extracellular matrix microenvironment.
MATN4 antibody is widely used in extracellular matrix, musculoskeletal, and developmental biology research. It is suitable for immunohistochemistry, ELISA, and western blotting to detect Matrilin-4 expression and distribution. This antibody supports studies of cartilage formation, joint pathology, and matrix protein interactions. In biomaterials research, MATN4 serves as a reference marker for cartilage scaffold design and regenerative tissue engineering.
Structurally, Matrilin-4 consists of vWFA domains that mediate protein-protein binding and a coiled-coil domain enabling tetramer formation. It is secreted via the classical ER-Golgi pathway and undergoes glycosylation for structural stability.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
