| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived mouse Mbd4 recombinant protein (Position: R238-D534) was used as the immunogen for the Mbd4 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
Mbd4 Antibody / Methyl-CpG-binding domain protein 4 is a anti-MBD4 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Flow cytometry (FACS), ELISA with listed reactivity in Mouse, Rat.
Key elements and design rationale
- Target: MBD4
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, FACS, ELISA
Biological background
Mbd4 is encoded by the MBD4 gene located on human chromosome 3q21.3. The protein contains two main functional domains: an N-terminal methyl-CpG-binding domain (MBD) that targets methylated DNA, and a C-terminal glycosylase domain that catalyzes base excision. Through these domains, Mbd4 directly interacts with DNA methyltransferases and participates in active DNA demethylation pathways. It also forms complexes with MLH1, a mismatch repair factor, integrating base excision repair with the mismatch repair system.
The Mbd4 antibody typically detects a 63 kilodalton protein on western blot. Mbd4 is highly expressed in proliferative tissues and cells undergoing DNA replication. Functionally, loss of Mbd4 leads to increased mutation frequency at CpG sites, predisposing cells to carcinogenesis. Experimental models show that Mbd4-deficient mice accumulate G:T mismatches and are more susceptible to DNA damage induced by oxidative stress or alkylating agents.
Beyond repair, Mbd4 plays an important role in apoptosis and gene silencing. It interacts with the tumor suppressor p53 and participates in transcriptional repression of methylated gene promoters. Dysregulation of Mbd4 expression or mutations in its glycosylase domain have been linked to colorectal cancer, glioma, and leukemia, where defective base excision repair contributes to genomic instability.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
