| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human MCEMP1 recombinant protein (Position: M1-Q183) was used as the immunogen for the MCEMP1 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
MCEMP1 Antibody / Mast cell-expressed membrane protein 1 is a anti-MCEMP1 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), ELISA with listed reactivity in Human, Mouse, Rat.
Key elements and design rationale
- Target: MCEMP1
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, ELISA
Biological background
Functionally, MCEMP1 antibody identifies a 177-amino-acid membrane glycoprotein featuring a short extracellular region, a single transmembrane domain, and a cytoplasmic tail containing an immunoreceptor tyrosine-based activation motif (ITAM). Upon phosphorylation, MCEMP1 can recruit SYK kinase and other signaling molecules, promoting cytokine production and cell activation.
The MCEMP1 gene is located on chromosome 19q13.4 and is expressed primarily in immune cells of the myeloid lineage, including mast cells, basophils, and macrophages. MCEMP1 expression increases during inflammation, allergic responses, and infection, suggesting a role in innate immune activation. It may also contribute to cytokine cross-talk between immune cells and epithelial barriers.
Pathologically, aberrant MCEMP1 expression is associated with allergic inflammation, asthma, and sepsis. Its upregulation in immune cells correlates with hyperinflammatory states, making it a potential biomarker for inflammatory disorders. Research using MCEMP1 antibody helps clarify its role in immune activation and signaling mechanisms.
MCEMP1 antibody is validated for western blotting, immunohistochemistry, and flow cytometry to detect immune cell membrane proteins.
Structurally, Mast cell-expressed membrane protein 1 contains a single-pass transmembrane segment and cytoplasmic ITAM motifs that drive downstream kinase signaling. This antibody aids in studies of MCEMP1's potential receptor functions and involvement in immune regulation.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
