| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human GANP/MCM3AP recombinant protein (Position: R1275-H1715) was used as the immunogen for the MCM3AP antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
MCM3AP Antibody / Minichromosome maintenance complex-binding protein 3-associated protein is a anti-MCM3AP Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat. Reported localization: Nuclear, cytoplasmic.
Key elements and design rationale
- Target: MCM3AP
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, ICC, IF, FACS, ELISA
Biological background
Functionally, MCM3AP antibody identifies a 200 kDa protein that interacts with the MCM3 subunit of the MCM helicase complex, which is essential for DNA replication initiation. MCM3AP acts as a replication checkpoint regulator, controlling helicase loading and preventing aberrant origin firing. Beyond replication, MCM3AP functions as a scaffold protein that anchors mRNA export factors to nuclear pore complexes through its association with ENY2 and PCID2. This dual involvement in DNA synthesis and mRNA trafficking underscores its central role in coordinating gene expression and genome maintenance.
The MCM3AP gene is located on chromosome 21q22.3 and encodes a large multidomain protein containing a histone acetyltransferase (HAT)-like region and a nuclear localization signal. The HAT-like domain acetylates replication factors, including MCM3, thereby modulating their chromatin association. MCM3AP also contributes to immunoglobulin gene hypermutation in germinal center B cells, reflecting its role in transcription-coupled mutagenesis. Its expression is elevated in proliferating lymphocytes, embryonic cells, and cancer tissues, where it supports rapid DNA replication and transcriptional activity.
In addition to its nuclear functions, MCM3AP has been implicated in mRNA surveillance and export. Through the TREX-2 complex, it connects RNA polymerase II transcription sites to nuclear pore channels, facilitating efficient export of mature mRNA to the cytoplasm. Loss of MCM3AP disrupts mRNA export, leading to nuclear accumulation of transcripts and impaired protein synthesis. Knockdown studies demonstrate that MCM3AP depletion results in DNA replication stress, S-phase arrest, and genomic instability.
MCM3AP antibody is widely used in research investigating DNA replication control, mRNA processing, and chromatin dynamics. Applications include western blotting, chromatin immunoprecipitation (ChIP), and confocal microscopy to analyze nuclear localization and replication factor interactions. In cancer studies, MCM3AP is explored as a potential biomarker of proliferation and genome integrity. It has been shown to enhance cell cycle progression and influence gene expression programs that support tumor growth and immune function.
Structurally, MCM3AP contains coiled-coil and zinc finger domains that mediate protein-protein interactions within the replication machinery and nuclear pore complexes. Post-translational modifications such as phosphorylation and acetylation regulate its activity during different cell cycle phases.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
