| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | A synthesized peptide derived from human MCM6 was used as the immunogen for the MCM6 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
MCM6 Antibody / Minichromosome maintenance complex component 6 is a anti-MCM6 Rabbit antibody Recombinant Rabbit Monoclonal clone 30M83 supplied in Liquid format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), Flow cytometry (FACS) with listed reactivity in Human, Mouse, Rat.
Key elements and design rationale
- Target: MCM6
- Antibody details: Rabbit, Recombinant Rabbit Monoclonal, clone 30M83, isotype Rabbit IgG
- Format: Liquid
- Applications (as listed): WB, IHC, ICC, IF, IP, FACS
Biological background
The MCM2 7 complex unwinds duplex DNA at replication origins, enabling the recruitment of DNA polymerases and synthesis of new strands. Research using MCM6 antibody has demonstrated that Minichromosome maintenance complex component 6 is required for helicase assembly and activity, forming part of the hexameric ring structure that encircles DNA. This function ensures faithful genome duplication during each cell cycle.
Studies with MCM6 antibody have revealed that expression of Minichromosome maintenance complex component 6 is tightly regulated by cell cycle cues. MCM6 accumulates during G1 phase, associates with chromatin at replication origins, and is activated in S phase. Its expression declines as cells exit the cycle, making MCM6 a marker of proliferative activity. Because of this, MCM6 is frequently used in pathology to assess tumor proliferation alongside markers such as Ki-67.
Aberrant regulation of MCM6 contributes to disease. Research using MCM6 antibody has shown that overexpression occurs in many cancers, where it supports uncontrolled DNA replication and cell cycle progression. Elevated MCM6 correlates with poor clinical outcomes, reflecting its role in sustaining proliferation. Conversely, reduced expression impairs DNA replication, leading to genomic instability. These findings highlight MCM6 as both a biomarker and a potential therapeutic target.
MCM6 antibody is applied in western blotting, immunohistochemistry, and flow cytometry. Western blotting detects endogenous protein levels, immunohistochemistry highlights nuclear localization in proliferating cells, and flow cytometry quantifies expression across cell populations. These applications make MCM6 antibody valuable in both basic and translational research.
By supplying validated MCM6 antibody reagents,
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Monoclonal antibodies provide a defined epitope recognition profile that can support consistent comparisons across experiments.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
