| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human MCM9 recombinant protein (Position: M1-L644) was used as the immunogen for the MCM9 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
MCM9 Antibody / Minichromosome maintenance complex component 9 is a anti-MCM9 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Flow cytometry (FACS), ELISA with listed reactivity in Human.
Key elements and design rationale
- Target: MCM9
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, FACS, ELISA
Biological background
Structurally, MCM9 is a 1,143-amino-acid nuclear protein of approximately 130 kilodaltons containing an AAA+ ATPase domain typical of MCM family helicases. Although it lacks intrinsic helicase activity, MCM9 interacts with MCM8 to form a functional complex that promotes DNA end resection and strand invasion during homologous recombination. It also associates with replication proteins such as RPA and RAD51, coordinating DNA repair with replication fork progression.
The MCM9 antibody is widely used in cell cycle, DNA repair, and cancer research to study replication licensing, recombination mechanisms, and genome maintenance. Western blot analysis detects a 130 kilodalton band corresponding to MCM9, while immunofluorescence shows distinct nuclear foci that colocalize with replication and repair markers. This antibody enables detailed analysis of DNA replication and damage response pathways.
Functionally, MCM9 is required for the recruitment of repair proteins to sites of DNA damage and for the restart of stalled replication forks. Mutations in MCM9 are associated with hereditary colorectal cancer syndromes and premature ovarian failure due to impaired DNA repair capacity. Loss of MCM9 function results in genomic instability and sensitivity to DNA-damaging agents. The MCM9 antibody provides a key reagent for investigating replication integrity, homologous recombination, and tumor suppression mechanisms.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
