| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human MCMBP recombinant protein (Position: E234-E534) was used as the immunogen for the MCMBP antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
MCMBP Antibody / MCM-binding protein is a anti-MCMBP Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunoprecipitation (IP), ELISA with listed reactivity in Human, Mouse, Rat.
Key elements and design rationale
- Target: MCMBP
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, IP, ELISA
Biological background
MCMBP is encoded by the MCMBP gene located on human chromosome 10q24.2. The protein is approximately 70 kilodaltons in size and associates with MCM subunits MCM2-MCM7. These interactions facilitate the transport of MCM complexes into the nucleus and their incorporation into pre-replication complexes at replication origins. MCMBP assists in maintaining replication fork integrity and prevents unscheduled replication events that could lead to genomic instability. It also contributes to the disassembly of replication machinery during mitotic exit.
The MCMBP antibody identifies the 70 kilodalton protein by western blot and reveals nuclear localization during S phase, with partial cytoplasmic presence in early G1. Immunofluorescence shows co-localization with replication foci, supporting its role in DNA synthesis regulation. Functional studies demonstrate that depletion of MCMBP causes replication stress, reduced origin firing, and accumulation of DNA damage markers such as gamma-H2AX. MCMBP interacts with replication factor C and proliferating cell nuclear antigen, integrating replication initiation with elongation control.
Beyond DNA replication, MCMBP is involved in maintaining chromosomal stability and has been linked to mitotic checkpoint regulation. Overexpression has been reported in certain cancers, including hepatocellular and colorectal carcinomas, where it may promote cell cycle progression. Conversely, its loss sensitizes cells to replication inhibitors, suggesting potential as a therapeutic target.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
